CDC25B Polyclonal antibody

CDC25B Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human and More (1)

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Publications

1

Cat no : 28109-1-AP

Synonyms

M-phase inducer phosphatase 2, M phase inducer phosphatase 2, EC:3.1.3.48, Cell division cycle 25 homolog B, CDC25HU2

---

Validation Data Gallery

• 

  WB analysis using 28109-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 28109-1-AP (CDC25B antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HepG2 using 28109-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 28109-1-AP (CDC25B antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 28109-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 28109-1-AP (CDC25B antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human ovary tumor using 28109-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 28109-1-AP (CDC25B antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human ovary tumor using 28109-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 28109-1-AP (CDC25B antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

---

Tested Applications

• Positive WB detected in: HepG2 cells, HeLa cells
• Positive IHC detected in: human ovary tumor tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 1 publications below
• IHC: See 1 publications below

Product Information

28109-1-AP targets CDC25B in WB, IHC, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Cited Reactivity: mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: CDC25B fusion protein Ag27138
• Full Name: cell division cycle 25 homolog B (S. pombe)
• Calculated Molecular Weight: 65 kDa
• Observed Molecular Weight: 60-70 kDa
• GenBank Accession Number: BC006395
• Gene Symbol: CDC25B
• Gene ID (NCBI): 994
• RRID: AB_2881063
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

CDC25B, also named as CDC25HU2, belongs to the MPI phosphatase family. It is a tyrosine protein phosphatase which functions as a dosage-dependent inducer of mitotic progression. CDC25B directly dephosphorylates CDK1 and stimulates its kinase activity. The three isoforms seem to have a different level of activity.

Publications

Species	Application	Title
mouse	WB,IHC	Cell Biol Toxicol; CircZNF609 promotes bladder cancer progression and inhibits cisplatin sensitivity via miR-1200/CDC25B pathway; Authors - Dexiang Feng