RASGRF1 Polyclonal antibody

• Featured Product
• KD/KO Validated

RASGRF1 Polyclonal Antibody for WB, IP, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

mouse, rat

Applications

WB, IP, IHC, ELISA

Conjugate

Unconjugated

Publications

3

Cat no : 12958-1-AP

Synonyms

CDC25, CDC25L, GNRP, GRF1, GRF55, H GRF55, PP13187, Ras GRF1, RAS-GRF, RASGRF1

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Validation Data Gallery

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  WB analysis of rat brain using 12958-1-AP

  rat brain tissue were subjected to SDS PAGE followed by western blot with 12958-1-AP (RASGRF1 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  IP experiment of rat brain using 12958-1-AP

  IP result of anti-RASGRF1 (IP:12958-1-AP, 4ug; Detection:12958-1-AP 1:600) with rat brain tissue lysate 1120 ug.

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  IHC staining of rat brain using 12958-1-AP

  Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 12958-1-AP (RASGRF1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse brain using 12958-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 12958-1-AP (RASGRF1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

• Positive WB detected in: rat brain tissue
• Positive IP detected in: rat brain tissue
• Positive IHC detected in: rat brain tissue, mouse brain tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 1 publications below
• WB: See 2 publications below
• IHC: See 1 publications below

Product Information

12958-1-AP targets RASGRF1 in WB, IP, IHC, ELISA applications and shows reactivity with mouse, rat samples.

• Tested Reactivity: mouse, rat
• Cited Reactivity: mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: RASGRF1 fusion protein Ag4072
• Full Name: Ras protein-specific guanine nucleotide-releasing factor 1
• Calculated Molecular Weight: 134 kDa
• Observed Molecular Weight: 130-140 kDa
• GenBank Accession Number: BC040275
• Gene Symbol: RASGRF1
• Gene ID (NCBI): 5923
• RRID: AB_2238126
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

RasGRF1, a member of calmodulin-activated guanine-nucleotide exchange factors (GEFs), highly expressed at the synaptic junctions of the central nervous system (CNS). It is similar to the Saccharomyces cerevisiae CDC25. RASGRF1 serves as an in vivo activator for H-RAS and members of the R-RAS and RAC subfamilies. Several studies have shown that RasGRF1 also binds to microtubules and participate in the regulation of neurite outgrowth. RasGRF1 has been implicated in neuronal excitability. The studies of the similar gene in mouse suggested that the Ras-GEF activity of this protein in brain can be activated by Ca2+ influx, muscarinic receptors, and G protein beta-gamma subunit. Mouse studies also indicated that the Ras-GEF signaling pathway mediated by this protein may be important for long-term memory.

Publications

Species	Application	Title
rat	WB,IHC	Aging (Albany NY); Upregulation of RasGRF1 ameliorates spatial cognitive dysfunction in mice after chronic cerebral hypoperfusion; Authors - Li-Jie Yang ; KD Validated
mouse	WB	J Agric Food Chem; Anthocyanins from Black Chokeberry (Aroniamelanocarpa Elliot) Delayed Aging-Related Degenerative Changes of Brain.; Authors - Jie Wei
		Leuk Lymphoma; Amplification of B cell receptor-Erk signaling by Rasgrf-1 over-expression in chronic lymphocytic leukemia.; Authors - Wei Liao