CDC23 Polyclonal antibody

CDC23 Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, ELISA

Conjugate

Unconjugated

Cat no : 10683-1-AP

Synonyms

ANAPC8, APC8, CDC23, Cyclosome subunit 8



Tested Applications

Positive WB detected inMOLT-4 cells, HEK-293 cells, Jurkat cells, K-562 cells, Caco-2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:3000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WBSee 1 publications below

Product Information

10683-1-AP targets CDC23 in WB, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen CDC23 fusion protein Ag1068
Full Name cell division cycle 23 homolog (S. cerevisiae)
Calculated Molecular Weight 68 kDa
Observed Molecular Weight 69 kDa
GenBank Accession NumberBC005258
Gene Symbol CDC23
Gene ID (NCBI) 8697
RRIDAB_2075008
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

CDC23(cell division cycle protein 23 homolog) is also named ANAPC8 and belongs to the APC8/CDC23 family. It is a subunit of the anaphase-promoting complex (APC), which functions at the metaphase-to-anaphase transition of the cell cycle and is regulated by spindle checkpoint proteins. It can be phosphorylated and the phosphorylation of Thr-562 occurs specifically during mitosis(PMID:19690332). It has 3 isoforms produced by alternative splicing with the molecular weight of 69 kDa, 17 kDa, and 56 kDa.

Protocols

Product Specific Protocols
WB protocol for CDC23 antibody 10683-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Adv Sci (Weinh)

CCDC68 Maintains Mitotic Checkpoint Activation by Promoting CDC20 Integration into the MCC

Authors - Qi Li