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CDC20 Recombinant antibody, PBS Only

CDC20 Uni-rAbTM Recombinant Antibody for WB, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

241925E12

Cat no : 84531-5-PBS

Synonyms

p55CDC, CDC20A, 241925E12

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using CDC20 Recombinant antibody (84531-5-RR)

    WB analysis using 84531-5-RR (same clone as 84531-5-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 84531-5-RR (CDC20 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84531-5-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 84531-5-RR

    Affinity and Kinetic Characterization of 84531-5-RR

    Biolayer interferometry (BLl) kinetic assays of 84531-5-RR against Human CDC20 were performed. The affinity constant is below 1 pM.

Product Information

84531-5-PBS targets CDC20 in WB, Indirect ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen CDC20 fusion protein Ag0295
Full Name cell division cycle 20 homolog (S. cerevisiae)
Calculated Molecular Weight 55 kDa
Observed Molecular Weight 55 kDa
GenBank Accession NumberBC001088
Gene Symbol CDC20
Gene ID (NCBI) 991
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

Cdc20 (Cell-division cycle protein 20 homologue) is also named as p55CDC and belongs to the WD repeat CDC20/Fizzy family. Cdc20 has important functions in chromosome segregation and mitotic exit. Cdc20 is the target of the spindle assembly checkpoint (SAC) and a key cofactor of the anaphase-promoting complex or cyclosome (APC/CCDC20) E3 ubiquitin ligase, thus regulating APC/C ubiquitin activity on specific substrates for their subsequent degradation by the proteasome (PMID: 28202332). The 55-kDa band corresponded to the size of Cdc20 (PMID: 22566641).