Anti-Human CD99 Rabbit Recombinant Antibody

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  FC experiment of human PBMCs using 98174-1-RR

  1x10^6 human PBMCs were surface stained with 0.25 ug Anti-Human CD99 Rabbit Recombinant Antibody (98174-1-RR, Clone:241737H1) or 0.25 ug Isotype Control, and PE-Conjugated Goat Anti-Rabbit IgG(H+L). Cells were not fixed.

• 

  FC experiment of human PBMCs using 98174-1-RR

  1x10^6 human PBMCs were surface stained with 0.25 ug Anti-Human CD99 Rabbit Recombinant Antibody (98174-1-RR, Clone:241737H1) (red) or 0.25 ug Isotype Control (blue), and PE-Conjugated Goat Anti-Rabbit IgG(H+L). Cells were not fixed.

• 

  Affinity and Kinetic Characterization of 98174-1-RR

  Biolayer interferometry (BLl) kinetic assays of 98174-1-RR against Human CD99 were performed. The affinity constant is 3.61 nM.

CD99 Recombinant Antibody for FC

Cat No. 98174-1-RR

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

FC

Synonyms

MIC2, E2 antigen, CD99 molecule, CD99 antigen, 241737H1

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Tested Applications

• Positive FC detected in: human PBMCs

Recommended dilution

Application	Dilution
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

98174-1-RR targets CD99 in FC applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: Recombinant Protein
• Full Name: CD99 molecule
• Calculated Molecular Weight: 19 kDa, 16 kDa, 17 kDa
• GenBank Accession Number: BC021620
• Gene Symbol: CD99
• Gene ID (NCBI): 4267
• RRID: AB_3672317
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purfication
• UNIPROT ID: P14209
• Storage Buffer: PBS with 0.09% sodium azide, pH 7.3.
• Storage Conditions: Store at 2 - 8°C. Stable for one year after shipment.

Background Information

CD99, also known as MIC2, is a heavily O-glycosylated transmembrane protein involved in T-cell adhesion processes and in spontaneous rosette formation with erythrocytes. CD99 is broadly distributed on many cell types, with particularly strong expression on human cortical thymocytes, Ewing's sarcoma cells and peripheral primitive neuroectodermal tumors (PMID: 9794396; 16984917). In normal cells, CD99 has been functionally implicated in cell adhesion, migration, apoptosis, differentiation, activation, and proliferation of lymphocytes and monocyte extravasation and transport of several transmembrane proteins (PMID: 16984917).