CD99 Recombinant antibody, PBS Only (Capture)

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  Sandwich ELISA standard curve of MP01403-3

  Sandwich ELISA standard curve of MP01403-3, Humam CD99 Recombinant Matched Antibody Pair - PBS only. 84580-3-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard RP02321. 84580-1-PBS was HRP conjugated as the detection antibody. Range: 0.313-20 ng/mL

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  Sample test of MP01403-3

  The mean CD99 concentration was determined to be 328.33 ng/mL in A549 cell extract based on a 2.5 mg/mL extract load and 96.35 ng/mL in A431 cell extract based on a 1.3 mg/mL extract load.

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  Sample test of MP01403-3

  HEK-293 cells were cultured in DMEM supplemented with 10% fetal bonive serum, 2.5 mM L-glutamine, 100 U/mL penicillin, and 100 μg/mL streptomycin sulfate. An aliquot of the cell culture supernate wasremoved, assayed for human CD99, and measured 1.59 ng/mL.

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  Sample test of MP01403-3

  Urine of six individual healthy human donors was measured. The human CD99 concentration of detected samples was determined to be 93.60 ng/mL with a range of 19.59 - 184.77 ng/mL.

CD99 {ptg:Brand} Recombinant Antibody for Sandwich ELISA, Indirect ELISA, Sample test

Cat No. 84580-3-PBS

Clone No.241736A10

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

Sandwich ELISA, Indirect ELISA, Sample test

Synonyms

MIC2, E2 antigen, CD99 molecule, CD99 antigen, 241736A10

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Product Information

84580-3-PBS targets CD99 as part of a matched antibody pair:

MP01403-3: 84580-3-PBS capture and 84580-1-PBS detection (validated in Sandwich ELISA)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: Recombinant Protein
• Full Name: CD99 molecule
• Calculated Molecular Weight: 19 kDa, 16 kDa, 17 kDa
• GenBank Accession Number: BC021620
• Gene Symbol: CD99
• Gene ID (NCBI): 4267
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: P14209
• Storage Buffer: PBS only , pH 7.3
• Storage Conditions: Store at -80°C.