CD99 Monoclonal antibody

CD99 Monoclonal Antibody for WB, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human

Applications

WB, ELISA

Conjugate

Unconjugated

Publications

1

CloneNo.

5B6A4

Cat no : 60354-1-Ig

Synonyms

12E7, CD99, CD99 antigen, CD99 molecule, E2 antigen, MIC2, MIC2X, MIC2Y, Protein MIC2, T cell surface glycoprotein E2

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Validation Data Gallery

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  WB analysis of human spleen using 60354-1-Ig

  human spleen tissue were subjected to SDS PAGE followed by western blot with 60354-1-Ig (CD99 Antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 60354-1-Ig

  HeLa cells were subjected to SDS PAGE followed by western blot with 60354-1-Ig (CD99 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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Tested Applications

• Positive WB detected in: human spleen tissue, HeLa cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:4000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 1 publications below

Product Information

60354-1-Ig targets CD99 in WB, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Cited Reactivity: human
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: CD99 fusion protein Ag19474
• Full Name: CD99 molecule
• Calculated Molecular Weight: 19 kDa, 16 kDa, 17 kDa
• Observed Molecular Weight: 32 kDa
• GenBank Accession Number: BC021620
• Gene Symbol: CD99
• Gene ID (NCBI): 4267
• RRID: AB_2881463
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein G purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

CD99, also known as MIC2, is a heavily O-glycosylated transmembrane protein involved in T-cell adhesion processes and in spontaneous rosette formation with erythrocytes. CD99 is broadly distributed on many cell types, with particularly strong expression on human cortical thymocytes, Ewing’s sarcoma cells and peripheral primitive neuroectodermal tumors (PMID: 9794396; 16984917). In normal cells, CD99 has been functionally implicated in cell adhesion, migration, apoptosis, differentiation, activation, and proliferation of lymphocytes and monocyte extravasation and transport of several transmembrane proteins (PMID: 16984917). CD99 displays two surface isoforms generated by alternative splicing: a long 32 kDa and a short 28 kDa form (PMID: 12368226).

Publications

Species	Application	Title
human	WB	Nat Struct Mol Biol; A novel class of microRNA-recognition elements that function only within open reading frames.; Authors - Kai Zhang