CD99 Polyclonal antibody

CD99 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human and More (1)

Applications

WB, IF, IHC, ELISA

Conjugate

Unconjugated

Publications

3

Cat no : 23079-1-AP

Synonyms

12E7, CD99, CD99 antigen, CD99 molecule, E2 antigen, MIC2, MIC2X, MIC2Y, Protein MIC2, T cell surface glycoprotein E2

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Validation Data Gallery

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  WB analysis using 23079-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 23079-1-AP (CD99 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of U-937 using 23079-1-AP

  U-937 cells were subjected to SDS PAGE followed by western blot with 23079-1-AP (CD99 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IHC staining of human pancreas using 23079-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas slide using 23079-1-AP (CD99 Antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human pancreas using 23079-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas slide using 23079-1-AP (CD99 Antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human testis using 23079-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis slide using 23079-1-AP (CD99 Antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human testis using 23079-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis slide using 23079-1-AP (CD99 Antibody) at dilution of 1:50 (under 40x lens).

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Tested Applications

• Positive WB detected in: Jurkat cells, U-937 cells, THP-1 cells
• Positive IHC detected in: human pancreas tissue, human testis tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunohistochemistry (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• IHC: See 3 publications below
• IF: See 1 publications below

Product Information

The immunogen of 23079-1-AP is CD99 Fusion Protein expressed in E. coli.

• Tested Reactivity: human
• Cited Reactivity: human, pig
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: CD99 fusion protein Ag19461
• Full Name: CD99 molecule
• Calculated Molecular Weight: 19 kDa, 16 kDa, 17 kDa
• Observed Molecular Weight: 28 kDa, 32 kDa
• GenBank Accession Number: BC021620
• Gene Symbol: CD99
• Gene ID (NCBI): 4267
• RRID: AB_2879205
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen Affinity purified
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

CD99, also known as MIC2, is a heavily O-glycosylated transmembrane protein involved in T-cell adhesion processes and in spontaneous rosette formation with erythrocytes. CD99 is broadly distributed on many cell types, with particularly strong expression on human cortical thymocytes, Ewing's sarcoma cells and peripheral primitive neuroectodermal tumors (PMID: 9794396; 16984917). In normal cells, CD99 has been functionally implicated in cell adhesion, migration, apoptosis, differentiation, activation, and proliferation of lymphocytes and monocyte extravasation and transport of several transmembrane proteins (PMID: 16984917). CD99 displays two surface isoforms generated by alternative splicing: a long 32 kDa and a short 28 kDa form (PMID: 12368226).

Publications

Species	Application	Title
pig	IHC,IF	J Anim Sci Biotechnol; Single-cell RNA-sequencing reveals the dynamic process and novel markers in porcine spermatogenesis.; Authors - Lingkai Zhang
human	IHC	Oncol Lett; Metastatic extraskeletal Ewing's sarcoma treated with trabectedin: A case report.; Authors - Jorge Hernando-Cubero
human	IHC	Cell Death Dis; KDM5B promotes tumorigenesis of Ewing sarcoma via FBXW7/CCNE1 axis.; Authors - Binbin Chen