CD98/SLC3A2 Monoclonal antibody, PBS Only

CD98/SLC3A2 Monoclonal Antibody for WB, IF, IHC, ELISA

Host / Isotype

Mouse / IgG2a

Reactivity

Human

Applications

WB, IF, IHC, ELISA

Conjugate

Unconjugated

CloneNo.

2B10F5

Cat no : 66883-1-PBS

Synonyms

4F2, 4F2 heavy chain antigen, 4F2HC, 4T2HC, CD98, CD98/SLC3A2, CD98HC, MDU1, NACAE, SLC3A2

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Validation Data Gallery

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  WB analysis using 66883-1-Ig (same clone as 66883-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 66883-1-Ig (CD98/SLC3A2 antibody) at dilution of 1:100000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Lamin B1 Monoclonal antibody (HRP-66095) as loading control. This data was developed using the same antibody clone with 66883-1-PBS in a different storage buffer formulation.

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  IHC staining of human renal cell carcinoma using 66883-1-Ig (same clone as 66883-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human renal cell carcinoma tissue slide using 66883-1-Ig (CD98/SLC3A2 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66883-1-PBS in a different storage buffer formulation.

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  IHC staining of human renal cell carcinoma using 66883-1-Ig (same clone as 66883-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human renal cell carcinoma tissue slide using 66883-1-Ig (CD98/SLC3A2 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66883-1-PBS in a different storage buffer formulation.

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  IHC staining of human colon cancer using 66883-1-Ig (same clone as 66883-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 66883-1-Ig (CD98/SLC3A2 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66883-1-PBS in a different storage buffer formulation.

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  IHC staining of human cervical cancer using 66883-1-Ig (same clone as 66883-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 66883-1-Ig (CD98/SLC3A2 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66883-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human cervical cancer using 66883-1-Ig (same clone as 66883-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 66883-1-Ig (CD98/SLC3A2 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66883-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human liver cancer using 66883-1-Ig (same clone as 66883-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 66883-1-Ig (CD98/SLC3A2 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66883-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human colon cancer using 66883-1-Ig (same clone as 66883-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 66883-1-Ig (CD98/SLC3A2 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66883-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human liver cancer using 66883-1-Ig (same clone as 66883-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 66883-1-Ig (CD98/SLC3A2 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66883-1-PBS in a different storage buffer formulation.

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  IF Staining of HeLa using 66883-1-Ig (same clone as 66883-1-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using CD98/SLC3A2 antibody (66883-1-Ig, Clone: 2B10F5 ) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66883-1-PBS in a different storage buffer formulation.

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Recommended dilution

Application	Dilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

66883-1-PBS targets CD98/SLC3A2 in WB, IF, IHC, ELISA applications and shows reactivity with Human samples.

• Tested Reactivity: Human
• Host / Isotype: Mouse / IgG2a
• Class: Monoclonal
• Type: Antibody
• Immunogen: CD98/SLC3A2 fusion protein Ag7301
• Full Name: solute carrier family 3 (activators of dibasic and neutral amino acid transport), member 2
• Calculated Molecular Weight: 68 kDa
• Observed Molecular Weight: 85-94 kDa, 120-130 kDa
• GenBank Accession Number: BC001061
• Gene Symbol: SLC3A2
• Gene ID (NCBI): 6520
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS only
• Storage Conditions: Store at -80°C.

Background Information

CD98 is a cell-surface heterodimer consisting of a heavy chain (CD98hc) and a light chain. CD98hc also interacts with certain integrin b-subunits to regulate cell migration, survival, proliferation, and adhesion/polarity. CD98hc is overexpressed on the cell surface of many cancers and increased CD98hc expression is associated with the development and progression of tumors.(PMID:25084765). This protein has 4 isoforms with the molecular mass of 58-71 kDa and can be detected 85-94 kDa due to glycosylation. The glycosylated CD98hc can links to a non-glycosylated light chain (~40 kDa) via a disulfide bond to form a heterodimeric CD98 antigen with molecular mass of 120-130 kDa (PMID: 14770309).