Various lysates were subjected to SDS PAGE followed by western blot with 84466-4-RR (CD94 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.
Various lysates were subjected to SDS PAGE followed by western blot with 84466-4-RR (CD94 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.
Affinity and Kinetic Characterization of 84466-4-RR
Biolayer interferometry (BLl) kinetic assays of 84466-4-RR against Human CD94 were performed. The affinity constant is 3.62 nM.
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PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage Conditions
Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.
Background Information
CD94, also known as KLRD1, is a membrane protein that is preferentially expressed on NK cells. CD94 can form disulfide-bonded heterodimers with NKG2 family members. The CD94/NKG2A heterodimer constitutes an inhibitory receptor that recognizes HLA-E.
Various lysates were subjected to SDS PAGE followed by western blot with 84466-4-RR (CD94 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.
AFFINITY Figures
Affinity and Kinetic Characterization of 84466-4-RR
Biolayer interferometry (BLl) kinetic assays of 84466-4-RR against Human CD94 were performed. The affinity constant is 3.62 nM.
The species listed in Tested Reactivity are in-house verified and applicable species. For unlisted species, please refer to the homology analysis of the immunogen sequence and related species. For rabbit polyclonal antibodies, homology >70% is recommended. For mouse monoclonal antibodies and rabbit recombinant antibodies, homology >90% is recommended. Generally, the higher the homology, the greater the applicability. However, there will be certain differences in protein expression in different species, tissues or cells. Therefore, the homology analysis results are for reference only and do not serve as a guarantee.
