CD90/Thy1 Recombinant antibody

CD90/Thy1 Uni-rAb^TM Recombinant Antibody for WB, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

rat

Applications

WB, IF/ICC, ELISA

Conjugate

Unconjugated

CloneNo.

241388E2

Cat no : 84206-3-RR

Synonyms

Thy-1 membrane glycoprotein, Thy-1 antigen, Thy-1, Thy1, CD90

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Validation Data Gallery

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  WB analysis using 84206-3-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 84206-3-RR (CD90/Thy1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  IF Staining of PC-12 using 84206-3-RR

  Immunofluorescent analysis of (4% PFA) fixed PC-12 cells using CD90/Thy1 antibody (84206-3-RR, Clone: 241388E2 ) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

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  Affinity and Kinetic Characterization of 84206-3-RR

  Biolayer interferometry (BLl) kinetic assays of 84206-3-RR against Rat CD90/Thy1 were performed. The affinity constant is 27.1 pM.

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Tested Applications

• Positive WB detected in: PC-12 cells, rat brain tissue
• Positive IF/ICC detected in: PC-12 cells

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 84206-3-RR is CD90/Thy1 Fusion Protein expressed in E. coli.

• Tested Reactivity: rat
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: Fusion Protein
• Full Name: Thy-1 cell surface antigen
• Calculated Molecular Weight: 18 kDa
• Observed Molecular Weight: 25 kDa
• GenBank Accession Number: NM_012673.2
• Gene Symbol: Thy1
• Gene ID (NCBI): 24832
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

CD90, also known as THY1, is a 25-35 kD protein that is expressed on 1-4% of human fetal liver cells, cord blood cells, and bone marrow cells. CD90 is one of the essential surface molecules expressed on human MSC from bone marrow and other sources. Activation of Thy-1 has been reported to promote T cell activation. It also affects numerous nonimmunologic biological processes, including cellular adhesion, neurite outgrowth, tumor growth, migration, and cell death.