CD90 Polyclonal antibody

CD90 Polyclonal Antibody for WB, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

mouse, rat

Applications

WB, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 31949-1-AP

Synonyms

Thy-1 membrane glycoprotein, Thy-1 antigen, Thy-1, Thy1

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Validation Data Gallery

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  WB analysis using 31949-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 31949-1-AP (CD90/Thy1 antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.

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  IF Staining of PC-12 using 31949-1-AP

  Immunofluorescent analysis of (4% PFA) fixed PC-12 cells using CD90/Thy1 antibody (31949-1-AP) at dilution of 1:200 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002).

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Tested Applications

• Positive WB detected in: PC-12 cells, mouse brain tissue, rat brain tissue
• Positive IF/ICC detected in: PC-12 cells

Recommended dilution

• Western Blot (WB): WB : 1:2000-1:12000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

31949-1-AP targets CD90 in WB, IF/ICC, ELISA applications and shows reactivity with mouse, rat samples.

• Tested Reactivity: mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Recombinant protein
• Full Name: Thy-1 cell surface antigen
• Calculated Molecular Weight: 18 kDa
• Observed Molecular Weight: 25 kDa
• GenBank Accession Number: NM_012673.2
• Gene Symbol: Thy1
• Gene ID (NCBI): 24832
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity Purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

CD90, also known as Thy1, is a 25-35 kDa protein that is expressed on 1-4% of human fetal liver cells, cord blood cells, and bone marrow cells. CD90 is one of the essential surface molecules expressed on human MSC from bone marrow and other sources. Activation of Thy-1 has been reported to promote T cell activation. It also affects numerous nonimmunologic biological processes, including cellular adhesion, neurite outgrowth, tumor growth, migration, and cell death.