CD90 Polyclonal antibody

CD90 Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat, pig

Applications

WB, ELISA

Conjugate

Unconjugated

Cat no : 27770-1-AP

Synonyms

THY1, CD90 / Thy1, Thy-1 antigen, Thy 1 membrane glycoprotein, Thy 1 cell surface antigen

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Validation Data Gallery

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  WB analysis using 27770-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 27770-1-AP (CD90 / Thy1 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 27770-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 27770-1-AP (CD90 / Thy1 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

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Tested Applications

• Positive WB detected in: human brain tissue, mouse brain tissue, rat brain tissue, pig brain tissue

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

27770-1-AP targets CD90 in WB, ELISA applications and shows reactivity with human, mouse, rat, pig samples.

• Tested Reactivity: human, mouse, rat, pig
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: CD90 fusion protein Ag24713
• Full Name: Thy-1 cell surface antigen
• Calculated Molecular Weight: 161 aa, 18 kDa
• Observed Molecular Weight: 28 kDa
• GenBank Accession Number: BC065559
• Gene Symbol: CD90/Thy1
• Gene ID (NCBI): 7070
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

CD90, also known as THY1, is a 25-35 kD protein that is expressed on 1-4% of human fetal liver cells, cord blood cells, and bone marrow cells. CD90 is one of the essential surface molecules expressed on human MSC from bone marrow and other sources. Activation of Thy-1 has been reported to promote T cell activation. It also affects numerous nonimmunologic biological processes, including cellular adhesion, neurite outgrowth, tumor growth, migration, and cell death.