CD90 Polyclonal antibody

CD90 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat, pig

Applications

WB, IHC, IF, ELISA

Conjugate

Unconjugated

Cat no : 27178-1-AP

Synonyms

THY1, CD90 / Thy1, Thy-1 antigen, Thy 1 membrane glycoprotein, Thy 1 cell surface antigen



Tested Applications

Positive WB detected infetal human brain tissue, mouse brain tissue, rat brain tissue, pig brain tissue
Positive IHC detected inhuman colon cancer tissue, human lung cancer tissue, mouse spleen tissue, mouse brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

IFSee 7 publications below

Product Information

27178-1-AP targets CD90 in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat, pig samples.

Tested Reactivity human, mouse, rat, pig
Cited Reactivityhuman, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen CD90 fusion protein Ag25603
Full Name Thy-1 cell surface antigen
Calculated Molecular Weight 161 aa, 18 kDa
Observed Molecular Weight26 kDa
GenBank Accession NumberBC065559
Gene Symbol CD90/Thy1
Gene ID (NCBI) 7070
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

CD90, also known as THY1, is a 25-35 kD protein that is expressed on 1-4% of human fetal liver cells, cord blood cells, and bone marrow cells. CD90 is one of the essential surface molecules expressed on human MSC from bone marrow and other sources. Activation of Thy-1 has been reported to promote T cell activation. It also affects numerous nonimmunologic biological processes, including cellular adhesion, neurite outgrowth, tumor growth, migration, and cell death.

Protocols

Product Specific Protocols
WB protocol for CD90 antibody 27178-1-APDownload protocol
IHC protocol for CD90 antibody 27178-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseIF

J Orthop Translat

Osteostaticytes: A novel osteoclast subset couples bone resorption and bone formation

Authors - Zhiyuan Wei
ratIF

Neurogastroenterol Motil

Construction and identification of an immortalized rat intestinal smooth muscle cell line.

Authors - Huiling Chen
ratIF

ACS Nano

Micronano Titanium Accelerates Mesenchymal Stem Cells Aging through the Activation of Senescence-Associated Secretory Phenotype

Authors - Xuan Li
mouseIF

Mol Immunol

Inhibition of cGAS-STING pathway by stress granules after activation of M2 macrophages by human mesenchymal stem cells against drug induced liver injury

Authors - Yao Wang
humanIF

Stem Cells

Nicotinamide riboside modulates HIF-1 signaling to maintain and enhance odontoblastic differentiation in human dental pulp stem cells

Authors - Peimeng Zhan
humanIF

Biochem Pharmacol

H3K18 lactylation-mediated VCAM1 expression promotes gastric cancer progression and metastasis via AKT-mTOR-CXCL1 axis

Authors - Yupeng Zhao