CD90 Polyclonal antibody
CD90 Polyclonal Antibody for WB, IHC, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat, pig
Applications
WB, IHC, IF, ELISA
Conjugate
Unconjugated
Cat no : 27178-1-AP
Synonyms
Validation Data Gallery
Tested Applications
Positive WB detected in | fetal human brain tissue, mouse brain tissue, rat brain tissue, pig brain tissue |
Positive IHC detected in | human colon cancer tissue, human lung cancer tissue, mouse spleen tissue, mouse brain tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:500-1:1000 |
Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
Published Applications
IF | See 7 publications below |
Product Information
27178-1-AP targets CD90 in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat, pig samples.
Tested Reactivity | human, mouse, rat, pig |
Cited Reactivity | human, mouse, rat |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Type | Antibody |
Immunogen | CD90 fusion protein Ag25603 |
Full Name | Thy-1 cell surface antigen |
Calculated Molecular Weight | 161 aa, 18 kDa |
Observed Molecular Weight | 26 kDa |
GenBank Accession Number | BC065559 |
Gene Symbol | CD90/Thy1 |
Gene ID (NCBI) | 7070 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Antigen affinity purification |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
CD90, also known as THY1, is a 25-35 kD protein that is expressed on 1-4% of human fetal liver cells, cord blood cells, and bone marrow cells. CD90 is one of the essential surface molecules expressed on human MSC from bone marrow and other sources. Activation of Thy-1 has been reported to promote T cell activation. It also affects numerous nonimmunologic biological processes, including cellular adhesion, neurite outgrowth, tumor growth, migration, and cell death.
Protocols
Product Specific Protocols | |
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WB protocol for CD90 antibody 27178-1-AP | Download protocol |
IHC protocol for CD90 antibody 27178-1-AP | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |
Publications
Species | Application | Title |
---|---|---|
J Orthop Translat Osteostaticytes: A novel osteoclast subset couples bone resorption and bone formation | ||
Neurogastroenterol Motil Construction and identification of an immortalized rat intestinal smooth muscle cell line. | ||
ACS Nano Micronano Titanium Accelerates Mesenchymal Stem Cells Aging through the Activation of Senescence-Associated Secretory Phenotype | ||
Mol Immunol Inhibition of cGAS-STING pathway by stress granules after activation of M2 macrophages by human mesenchymal stem cells against drug induced liver injury | ||
Stem Cells Nicotinamide riboside modulates HIF-1 signaling to maintain and enhance odontoblastic differentiation in human dental pulp stem cells | ||
Biochem Pharmacol H3K18 lactylation-mediated VCAM1 expression promotes gastric cancer progression and metastasis via AKT-mTOR-CXCL1 axis |