CD90 Recombinant antibody

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  WB analysis using 84180-1-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 84180-1-RR (CD90 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  Affinity and Kinetic Characterization of 84180-1-RR

  Biolayer interferometry (BLl) kinetic assays of 84180-1-RR against Human CD90 were performed. The affinity constant is 0.383 nM.

CD90 Uni-rAb^TM Recombinant Antibody for WB, ELISA

Cat No. 84180-1-RR

Clone No.241290B1

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat, pig

Applications

WB, ELISA

Synonyms

THY1, CD90 / Thy1, 241290B1, Thy 1 membrane glycoprotein, Thy 1 cell surface antigen

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Tested Applications

• Positive WB detected in: SH-SY5Y cells, PC-12 cells, EL-4 cells, mouse brain tissue, rat brain tissue, pig brain tissue, fetal human brain tissue

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

84180-1-RR targets CD90 in WB, ELISA applications and shows reactivity with human, mouse, rat, pig samples.

• Tested Reactivity: human, mouse, rat, pig
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: Recombinant Protein
• Full Name: Thy-1 cell surface antigen
• Calculated Molecular Weight: 161 aa, 18 kDa
• Observed Molecular Weight: 25-35 kDa
• GenBank Accession Number: BC065559
• Gene Symbol: CD90/Thy1
• Gene ID (NCBI): 7070
• RRID: AB_3671735
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: P04216
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

CD90, also known as THY1, is a 25-35 kDa protein that is expressed on 1-4% of human fetal liver cells, cord blood cells, and bone marrow cells. CD90 is one of the essential surface molecules expressed on human MSC from bone marrow and other sources. Activation of Thy-1 has been reported to promote T cell activation. It also affects numerous nonimmunologic biological processes, including cellular adhesion, neurite outgrowth, tumor growth, migration, and cell death.