CD9 Recombinant antibody

CD9 Uni-rAb^TM Recombinant Antibody for IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

IF/ICC, ELISA

Conjugate

Unconjugated

CloneNo.

240830A6

Cat no : 84142-2-RR

Synonyms

CD9 antigen, BTCC 1, BA2, 5H9 antigen, 5H9

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Validation Data Gallery

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  IF Staining of MCF-7 using 84142-2-RR

  Immunofluorescent analysis of (4% PFA) fixed MCF-7 cells using CD9 antibody (84142-2-RR, Clone: 240830A6 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2).

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  Affinity and Kinetic Characterization of 84142-2-RR

  Biolayer interferometry (BLl) kinetic assays of 84142-2-RR against Human CD9 were performed. The affinity constant is 1.57 nM.

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Tested Applications

• Positive IF/ICC detected in: MCF-7 cells

Recommended dilution

• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 84142-2-RR is CD9 Fusion Protein expressed in E. coli.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: Fusion Protein
• Full Name: CD9 molecule
• Calculated Molecular Weight: 25 kDa
• Observed Molecular Weight: 23-30 kDa
• GenBank Accession Number: NM_001769.4
• Gene Symbol: CD9
• Gene ID (NCBI): 928
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purfication
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

The cell-surface molecule CD9, a member of the transmembrane-4 superfamily, interacts with the integrin family and other membrane proteins and is postulated to participate in cell migration and adhesion. Expression of CD9 enhances membrane fusion between muscle cells and promotes viral infection in some cells (PMID:10459022). It is often used as a mesenchymal stem cell marker (PMID:18005405). The CD9 antigen appears to be a 227-amino acid molecule with four hydrophobic domains and one N-glycosylation site (PMID: 1840589). This antibody detects bands of 23-30 kDa, which may be due to the difference in glycosylations (PMID: 8701996).