CD9 Recombinant antibody, PBS Only

CD9 Uni-rAb^TM Recombinant Antibody for WB, IHC, IF/ICC, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF/ICC, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

5K14

Cat no : 82105-1-PBS

Synonyms

CD9 antigen, BTCC 1, BA2, 5K14, 5H9 antigen

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Validation Data Gallery

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  WB analysis using 82105-1-RR (same clone as 82105-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 82105-1-RR (CD9 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 82105-1-PBS in a different storage buffer formulation.

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  WB analysis of human urine exosomes using 82105-1-RR (same clone as 82105-1-PBS)

  human urine and human urine-derived exosomes were subjected to SDS PAGE followed by western blot with 82105-1-RR (CD9 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 82105-1-PBS in a different storage buffer formulation.

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  IHC staining of human lung cancer using 82105-1-RR (same clone as 82105-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 82105-1-RR (CD9 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 82105-1-PBS in a different storage buffer formulation.

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  IHC staining of human lung cancer using 82105-1-RR (same clone as 82105-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 82105-1-RR (CD9 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 82105-1-PBS in a different storage buffer formulation.

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  IHC staining of human endometrial cancer using 82105-1-RR (same clone as 82105-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human endometrial cancer tissue slide using 82105-1-RR (CD9 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 82105-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human endometrial cancer using 82105-1-RR (same clone as 82105-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human endometrial cancer tissue slide using 82105-1-RR (CD9 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 82105-1-PBS in a different storage buffer formulation.

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  IF Staining of MCF-7 using 82105-1-RR (same clone as 82105-1-PBS)

  Immunofluorescent analysis of (-20°C Methanol) fixed MCF-7 cells using CD9 antibody (82105-1-RR, Clone: 5K14 ) at dilution of 1:1000 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 82105-1-PBS in a different storage buffer formulation.

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  Affinity and Kinetic Characterization of 82105-1-RR

  Biolayer interferometry (BLl) kinetic assays of 82105-1-RR against Human CD9 were performed. The affinity constant is 36.3 pM.

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Product Information

82105-1-PBS targets CD9 in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: CD9 fusion protein Ag14546
• Full Name: CD9 molecule
• Calculated Molecular Weight: 228 aa, 25 kDa
• Observed Molecular Weight: 23-27 kDa
• GenBank Accession Number: BC011988
• Gene Symbol: CD9
• Gene ID (NCBI): 928
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

CD9, also known as Tspan-29, p24 or MIC3, is a member of the tetraspanin superfamily (PMID: 1879540). It is expressed on a large variety of hematopoietic and non-hematopoietic cells, such as stromal cells, megakaryocytes, platelets, B and T lymphocytes, dendritic cells, endothelial cells, mast cells, eosinophils, and basophils (PMID: 30356731). CD9 interacts with the integrin family and other membrane proteins, and is involved in cell adhesion, cell motility and tumor metastasis (PMID: 8478605; 21428940; 25805926). Expression of CD9 enhances membrane fusion between muscle cells and support myotube maintenance (PMID:10459022). On oocytes, CD9 is hypothesized to play a role in fertilization of mammals (PMID: 25536312).