PE Anti-Mouse CD8a (5H10-1)

CD8a Monoclonal Antibody for FC

Host / Isotype

Rat / IgG2b, lambda

Reactivity

Mouse

Applications

FC

Conjugate

PE Fluorescent Dye

CloneNo.

5H10-1

Cat no : PE-65205

Synonyms

BB154331, CD8 antigen, alpha chain, Cd8a, Ly 2, Ly 35, Ly B, Lyt 2



Tested Applications

Positive FC detected inC57BL/6 mouse splenocytes

Recommended dilution

ApplicationDilution
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

PE-65205 targets CD8a in FC applications and shows reactivity with Mouse samples.

Tested Reactivity Mouse
Host / Isotype Rat / IgG2b, lambda
Class Monoclonal
Type Antibody
Immunogen Concanavalin A-stimulated BALB/c splenic T cells
Full Name CD8 antigen, alpha chain
GenBank Accession NumberBC030679
Gene Symbol Cd8a
Gene ID (NCBI) 12525
Conjugate PE Fluorescent Dye
Excitation/Emission Maxima Wavelengths496 nm, 565 nm / 578 nm
Form Liquid
Purification MethodAffinity purification
Storage Buffer PBS with 0.1% sodium azide and 0.5% BSA.
Storage ConditionsStore at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

Background Information

CD8 is a transmembrane glycoprotein composed of two disulfide-linked chains. It can be present as a homodimer of CD8α or as a heterodimer of CD8α and CD8β (PMID: 3264320; 8253791). CD8 is found on most thymocytes. The majority of class I-restricted T cells express mostly the CD8αβ heterodimer while CD8αα homodimers alone have been found on some gut intraepithelial T cells , on some T cell receptor (TCR) γδ T cells and on NK cells (PMID: 2111591; 1831127; 8420975). CD8 acts as a co-receptor that binds to MHC class-I and participates in cytotoxic T cell activation (PMID: 8499079). During T cell development, CD8 is required for positive selection of CD4-/CD8+ T cells (PMID: 1968084).

Protocols

Product Specific Protocols
FC protocol for PE CD8a antibody PE-65205Download protocol
Standard Protocols
Click here to view our Standard Protocols