CoraLite® Plus 488-conjugated CD8a Monoclonal antibody

CD8a Monoclonal Antibody for IF-P

Host / Isotype

Mouse / IgG1

Reactivity

human

Applications

IHC, IF-P

Conjugate

CoraLite® Plus 488 Fluorescent Dye

Publications

3

CloneNo.

1G2B10

Cat no : CL488-66868

Synonyms

CD8, 1G2B10, p32, MAL, Leu2

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Validation Data Gallery

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  IF Staining of human tonsillitis using CL488-66868

  Immunofluorescent analysis of (4% PFA) fixed human tonsillitis tissue using CoraLite® Plus 488 CD8a antibody (CL488-66868, Clone: 1G2B10 ) at dilution of 1:200, CoraLite®594 CD11c/Integrin Alpha X antibody (CL594-60258, Clone: 2F1C10, red).

• 

  IF Staining of human tonsillitis using CL488-66868

  Immunofluorescent analysis of (4% PFA) fixed human tonsillitis tissue using CoraLite® Plus 488 CD8a antibody (CL488-66868, Clone: 1G2B10 ) at dilution of 1:200.

• 

  IF Staining of human tonsillitis using CL488-66868

  Immunofluorescent analysis of (4% PFA) fixed human tonsillitis tissue using CoraLite® Plus 488 CD8a antibody (CL488-66868, Clone: 1G2B10 ) at dilution of 1:200.

• 

  IF Staining of human tonsillitis using CL488-66868

  Immunofluorescent analysis of (4% PFA) fixed human tonsillitis tissue using CL488-66868 (CD8a antibody) at dilution of 1:100.

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Tested Applications

• Positive IF-P detected in: human tonsillitis tissue

Recommended dilution

• Immunofluorescence (IF)-P: IF-P : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• IHC: See 1 publications below
• IF: See 1 publications below

Product Information

CL488-66868 targets CD8a in IHC, IF-P applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: CD8a fusion protein Ag11111
• Full Name: CD8a molecule
• Calculated Molecular Weight: 235 aa, 26 kDa
• GenBank Accession Number: BC025715
• Gene Symbol: CD8A
• Gene ID (NCBI): 925
• RRID: AB_2883388
• Conjugate: CoraLite® Plus 488 Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 493 nm / 522 nm
• Form: Liquid
• Purification Method: Protein G purification
• Storage Buffer: PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
• Storage Conditions: Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

CD8 is a transmembrane glycoprotein that is predominantly expressed on the surface of cytotoxic T cells, and can also be found on natural killer cells, cortical thymocytes, and dendritic cells. CD8 serves as a co-receptor for the T cell receptor (TCR). Both CD8 and TCR recognize antigens displayed by an antigen presenting cell (APC) in the context of class I MHC molecules. CD8 plays a role in T cell development and activation of mature T cells. This antibody is raised against 23-183 aa of human CD8a, the alpha chain of CD8 molecule, and is conjugated with CL488, Ex/Em 488 nm/515 nm.

Publications

Species	Application	Title
	IHC	FASEB J; Tertiary lymphoid structures: Associated multiple immune cells and analysis their formation in hepatocellular carcinoma; Authors - Ye Nie
mouse	IF	J Cardiovasc Pharmacol; Treg/Th17 Ratio Regulation May Play an Important Role in Epigallocatechin-3-Gallate-Mediated Attenuation of Increased Afterload-Induced Cardiac Hypertrophy; Authors - Min Luo
		J Ethnopharmacol; Huangqin Tang Alleviates Colitis-associated Colorectal Cancer via Amino Acids Homeostasisand PI3K/AKT/mTOR Pathway Modulation; Authors - Dunfang Wang