CoraLite® Plus 405 Anti-Rat CD8a (OX-8)

CD8a Monoclonal Antibody for FC

Host / Isotype

Mouse / IgG1, kappa

Reactivity

Rat

Applications

FC

Conjugate

CoraLite® Plus 405 Fluorescent Dye

CloneNo.

OX-8

Cat no : CL405-65180

Synonyms

Cd8a, CD8a molecule

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Validation Data Gallery

• 

  FC experiment of wistar rat splenocytes using CL405-65180

  1X10^6 Wistar rat splenocytes were surface stained with 0.5 ug CoraLite® Plus 405 Anti-Rat CD8a (CL405-65180, Clone: OX-8) or 0.5 ug Control Antibody. Cells were not fixed.

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Tested Applications

• Positive FC detected in: Wistar rat splenocytes

Recommended dilution

Application	Dilution
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

CL405-65180 targets CD8a in FC applications and shows reactivity with Rat samples.

• Tested Reactivity: Rat
• Host / Isotype: Mouse / IgG1, kappa
• Class: Monoclonal
• Type: Antibody
• Immunogen: High-molecular-weight rat thymocyte glycoproteins
• Full Name: CD8a molecule
• GenBank Accession Number: BC088126
• Gene Symbol: Cd8a
• Gene ID (NCBI): 24930
• Conjugate: CoraLite® Plus 405 Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 399 nm / 422 nm
• Form: Liquid
• Purification Method: Purified by protein-A affinity chromatography
• Storage Buffer: PBS with 0.09% sodium azide.
• Storage Conditions: Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

Background Information

CD8 is a transmembrane glycoprotein composed of two disulfide-linked chains. It can be present as a homodimer of CD8α or as a heterodimer of CD8α and CD8β (PMID: 3264320; 8253791). CD8 is found on most thymocytes. The majority of class I-restricted T cells express mostly the CD8αβ heterodimer while CD8αα homodimers alone have been found on some gut intraepithelial T cells , on some T cell receptor (TCR) γδ T cells and on NK cells (PMID: 2111591; 1831127; 8420975). CD8 acts as a co-receptor that binds to MHC class-I and participates in cytotoxic T cell activation (PMID: 8499079). During T cell development, CD8 is required for positive selection of CD4-/CD8+ T cells (PMID: 1968084).