Anti-Human CD8a (RPA-T8)

CD8a Monoclonal Antibody for FC

Host / Isotype

Mouse / IgG1, kappa

Reactivity

human and More (1)

Applications

FC

Conjugate

Unconjugated

Publications

4

CloneNo.

RPA-T8

Cat no : 65144-1-Ig

Synonyms

CD8, RPA-T8, p32, MAL, Leu2

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Validation Data Gallery

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  FC experiment of human PBMCs using 65144-1-Ig

  1x10^6 human PBMCs were surface stained with 0.2 ug Anti-Human CD8a (RPA-T8) (65144-1-Ig) or 0.2 ug Mouse IgG1 Isotype Control and CoraLite®647-conjugated F(ab')2 Fragment Goat Anti-Mouse IgG (H+L) (SA00014-8). Cells were then stained with 5 ul CoraLite® Plus 488 Anti-Human CD3 (CL488-65151). Cells were not fixed. Lymphocytes were gated.

• 

  FC experiment of human PBMCs using 65144-1-Ig

  1x10^6 human PBMCs were surface stained with 0.2 ug Anti-Human CD8a (RPA-T8) (65144-1-Ig) or 0.2 ug Mouse IgG1 Isotype Control and CoraLite®647-conjugated F(ab')2 Fragment Goat Anti-Mouse IgG (H+L) (SA00014-8). Cells were not fixed. Lymphocytes were gated.

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Tested Applications

• Positive FC detected in: human PBMCs

Recommended dilution

Application	Dilution
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 1 publications below
• IHC: See 2 publications below

Product Information

65144-1-Ig targets CD8a in FC applications and shows reactivity with human samples.

• Tested Reactivity: human
• Cited Reactivity: human, pig
• Host / Isotype: Mouse / IgG1, kappa
• Class: Monoclonal
• Type: Antibody
• Immunogen: N/A
• Full Name: CD8a molecule
• Calculated Molecular Weight: 235 aa, 26 kDa
• GenBank Accession Number: BC025715
• Gene Symbol: CD8A
• Gene ID (NCBI): 925
• RRID: AB_2918430
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Affinity purification
• Storage Buffer: PBS with 0.09% sodium azide.
• Storage Conditions: Store at 2-8°C. Stable for one year after shipment.

Background Information

CD8 is a transmembrane glycoprotein composed of two disulfide-linked chains. It can be present as a homodimer of CD8α or as a heterodimer of CD8α and CD8β (PMID: 3264320; 8253791). CD8 is found on most thymocytes. The majority of class I-restricted T cells express mostly the CD8αβ heterodimer while CD8αα homodimers alone have been found on some gut intraepithelial T cells , on some T cell receptor (TCR) γδ T cells and on NK cells (PMID: 2111591; 1831127; 8420975). CD8 acts as a co-receptor that binds to MHC class-I and participates in cytotoxic T cell activation (PMID: 8499079). During T cell development, CD8 is required for positive selection of CD4-/CD8+ T cells (PMID: 1968084).

Publications

Species	Application	Title
human	WB	Cancers (Basel); Inhibition of DCLK1 with DCLK1-IN-1 Suppresses Renal Cell Carcinoma Invasion and Stemness and Promotes Cytotoxic T-Cell-Mediated Anti-Tumor Immunity.; Authors - Ling Ding
human	IHC	Front Oncol; Comprehensive Characterization of Immune Landscape Based on Epithelial-Mesenchymal Transition Signature in OSCC: Implication for Prognosis and Immunotherapy.; Authors - Si-Yuan Zhang
human	IHC	Front Oncol; Typing and modeling of hepatocellular carcinoma based on disulfidptosis-related amino acid metabolism genes for predicting prognosis and guiding individualized treatment; Authors - Xuenuo Chen
pig		J Virol; Combinational Deletions of MGF360-9L and MGF505-7R Attenuated Highly Virulent African Swine Fever Virus and Conferred Protection against Homologous Challenge.; Authors - Mingyang Ding