FITC Plus Anti-Human CD86 (BU63) Rabbit Recombinant Antibody

CD86 Recombinant Antibody for FC

Host / Isotype

Rabbit / IgG

Reactivity

Human

Applications

FC

Conjugate

FITC Plus Fluorescent Dye

CloneNo.

BU63

Cat no : FITC-65514

Synonyms

Activation B7 2 antigen, B7 2, B70

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Validation Data Gallery

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  FC experiment of human PBMCs using FITC-65514

  1x10^6 human PBMCs were surface stained with 5 ul FITC Plus Anti-Human CD86 Rabbit Recombinant Antibody (FITC-65514, Clone:BU63) or FITC-conjugated Rabbit IgG control PolyAb (FITC-30000). Cells were not fixed. Cells were treated with FC Receptor Block prior to staining. Monocytes were gated.

• 

  FC experiment of human PBMCs using FITC-65514

  1x10^6 human PBMCs were surface stained with 5 ul FITC Plus Anti-Human CD86 Rabbit Recombinant Antibody (FITC-65514, Clone:BU63) or FITC-conjugated Rabbit IgG control PolyAb (FITC-30000). Cells were not fixed. Cells were treated with FC Receptor Block prior to staining. Monocytes were gated.

• 

  FC experiment of human PBMCs using FITC-65514

  1x10^6 human PBMCs were surface stained with 5 ul FITC Plus Anti-Human CD86 Rabbit Recombinant Antibody (FITC-65514, Clone:BU63) or 5 ul FITC Plus Anti-Human CD86 Mouse Monoclonal Antibody (FITC-65165, Clone:BU63). Cells were not fixed. Cells were treated with FC Receptor Block prior to staining. Monocytes were gated.

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Tested Applications

• Positive FC detected in: human PBMCs

Recommended dilution

Application	Dilution
This reagent has been pre-titrated and tested for flow cytometric analysis. The suggested use of this reagent is 5 µl per 10^6 cells in a 100 µl suspension or 5 µl per 100 µl of whole blood.
Sample-dependent, Check data in validation data gallery.

Product Information

FITC-65514 targets CD86 in FC applications and shows reactivity with Human samples.

• Tested Reactivity: Human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: B-lymphoblastoid cell line ARH 77
• Full Name: CD86 molecule
• Calculated Molecular Weight: 329 aa, 38 kDa
• GenBank Accession Number: BC040261
• Gene Symbol: CD86
• Gene ID (NCBI): 942
• Conjugate: FITC Plus Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 495 nm / 524 nm
• Form: Liquid
• Purification Method: Affinity purification
• Storage Buffer: PBS with 0.09% sodium azide and 0.5% BSA.
• Storage Conditions: Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

Background Information

CD86 (also known as B7-2) is a costimulatory molecule belonging to the immunoglobulin superfamily. Primarily expressed on antigen-presenting cells (APCs), including B cells, dendritic cells, and macrophages, CD86 is the ligand for two proteins at the cell surface of T cells, CD28 antigen and CTLA-4. Binding of CD86 with CD28 antigen is a costimulatory signal for activation of the T-cell. Binding of CD86 with CTLA-4 negatively regulates T-cell activation and diminishes the immune response. (PMID: 7513726; 1847722; 11029388; 27591335)