FITC Plus Anti-Mouse CD86 (GL1)
CD86 Monoclonal Antibody for FC
Host / Isotype
Rat / IgG2a, kappa
Reactivity
Mouse and More (1)
Applications
IF, FC
Conjugate
FITC Plus Fluorescent Dye
CloneNo.
GL1
Cat no : FITC-65068
Synonyms
Validation Data Gallery
Tested Applications
Positive FC detected in | LPS treated mouse splenocytes |
Recommended dilution
Application | Dilution |
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This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
Published Applications
IF | See 1 publications below |
FC | See 7 publications below |
Product Information
FITC-65068 targets CD86 in IF, FC applications and shows reactivity with Mouse samples.
Tested Reactivity | Mouse |
Cited Reactivity | mouse |
Host / Isotype | Rat / IgG2a, kappa |
Class | Monoclonal |
Type | Antibody |
Immunogen | LPS-activated CBA/Ca mouse spleen B cells |
Full Name | CD86 antigen |
GenBank Accession Number | BC013807 |
Gene Symbol | Cd86 |
Gene ID (NCBI) | 12524 |
RRID | AB_2883758 |
Conjugate | FITC Plus Fluorescent Dye |
Excitation/Emission Maxima Wavelengths | 495 nm / 524 nm |
Form | Liquid |
Purification Method | Affinity purification |
Storage Buffer | PBS with 0.09% sodium azide. |
Storage Conditions | Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment. |
Background Information
CD86 (also known as B7-2) is a costimulatory molecule belonging to the immunoglobulin superfamily. Primarily expressed on antigen-presenting cells (APCs), including B cells, dendritic cells, and macrophages, CD86 is the ligand for two proteins at the cell surface of T cells, CD28 antigen and CTLA-4. Binding of CD86 with CD28 antigen is a costimulatory signal for activation of the T-cell. Binding of CD86 with CTLA-4 negatively regulates T-cell activation and diminishes the immune response. (PMID: 7513726; 1847722; 11029388; 27591335)
Protocols
Product Specific Protocols | |
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FC protocol for FITC Plus CD86 antibody FITC-65068 | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |
Publications
Species | Application | Title |
---|---|---|
J Control Release Programmed antigen capture-harnessed dendritic cells by margination-hitchhiking lung delivery | ||
Cell Rep Mechanotransduction in response to ECM stiffening impairs cGAS immune signaling in tumor cells | ||
Carbohydr Polym Chemical structure and anti-inflammatory activity of a branched polysaccharide isolated from Phellinus baumii. | ||
Mol Neurobiol HPGD: An Intermediate Player in Microglial Polarization and Multiple Sclerosis Regulated by Nr4a1 | ||
Front Bioeng Biotechnol Vascular endothelial growth factor attenuates neointimal hyperplasia of decellularized small-diameter vascular grafts by modulating the local inflammatory response | ||
Cancer Immunol Immunother A nanotherapeutic system for gastric cancer suppression by synergistic chemotherapy and immunotherapy based on iPSCs and DCs exosomes |