CD86 Recombinant antibody, PBS Only

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  WB analysis using 83523-4-RR (same clone as 83523-4-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 83523-4-RR (CD86 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83523-4-PBS in a different storage buffer formulation.

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  IHC staining of human ovary cancer using 83523-4-RR (same clone as 83523-4-PBS)

  Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 83523-4-RR (CD86 antibody) at dilution of 1:300 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83523-4-PBS in a different storage buffer formulation.

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  IHC staining of human ovary cancer using 83523-4-RR (same clone as 83523-4-PBS)

  Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 83523-4-RR (CD86 antibody) at dilution of 1:300 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83523-4-PBS in a different storage buffer formulation.

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  IF Staining of Raji using 83523-4-RR (same clone as 83523-4-PBS)

  Immunofluorescent analysis of (4% PFA) fixed Raji cells using CD86 antibody (83523-4-RR, Clone: 240429G12 ) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 83523-4-PBS in a different storage buffer formulation.

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  Affinity and Kinetic Characterization of 83523-4-RR

  Biolayer interferometry (BLl) kinetic assays of 83523-4-RR against Human CD86 were performed. The affinity constant is 0.766 nM.

CD86 Recombinant Antibody for WB, IHC, IF/ICC, ELISA

Cat No. 83523-4-PBS

Clone No.240429G12

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF/ICC, ELISA

Synonyms

240429G12, Activation B7 2 antigen, Activation B7-2 antigen, B7 2, B70

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Product Information

83523-4-PBS targets CD86 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: CD86 fusion protein Eg0966
• Full Name: CD86 molecule
• Calculated Molecular Weight: 329 aa, 38 kDa
• Observed Molecular Weight: 70 kDa
• GenBank Accession Number: NM_175862
• Gene Symbol: CD86
• Gene ID (NCBI): 942
• ENSEMBL Gene ID: ENSG00000114013
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purfication
• UNIPROT ID: P42081
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

CD86 (also known as B7.2) is a costimulatory molecule belonging to the immunoglobulin superfamily. Primarily expressed on antigen-presenting cells (APCs), including B cells, dendritic cells, and macrophages, CD86 is the ligand for two proteins at the cell surface of T cells, CD28 antigen and cytotoxic T-lymphocyte-associated protein 4. Binding of CD86 with CD28 antigen is a costimulatory signal for activation of the T-cell. Binding of CD86 with cytotoxic T-lymphocyte-associated protein 4 negatively regulates T-cell activation and diminishes the immune response.