CD86 Recombinant antibody

CD86 Uni-rAb^TM Recombinant Antibody for IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Mouse

Applications

IF/ICC, ELISA

Conjugate

Unconjugated

CloneNo.

230476B2

Cat no : 83213-1-RR

Synonyms

B7-2, B70, B7.2, B7 2, B7

---

Validation Data Gallery

• 

  IF Staining of RAW 264.7 using 83213-1-RR

  Immunofluorescent analysis of (4% PFA) fixed RAW 264.7 cells using CD86 antibody (83213-1-RR, Clone: 230476B2 ) at dilution of 1:250 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

• 

  Affinity and Kinetic Characterization of 83213-1-RR

  Biolayer interferometry (BLl) kinetic assays of 83213-1-RR against Mouse CD86 were performed. The affinity constant is 0.747 nM.

---

Tested Applications

• Positive IF/ICC detected in: RAW 264.7 cells

Recommended dilution

• Immunofluorescence (IF)/ICC: IF/ICC : 1:125-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

83213-1-RR targets CD86 in IF/ICC, ELISA applications and shows reactivity with Mouse samples.

• Tested Reactivity: Mouse
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: Recombinant protein
• Full Name: CD86 antigen
• Calculated Molecular Weight: 35 kDa
• GenBank Accession Number: NM_019388
• Gene Symbol: Cd86
• Gene ID (NCBI): 12524
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

CD86 (also known as B7.2) is a costimulatory molecule belonging to the immunoglobulin superfamily. Primarily expressed on antigen-presenting cells (APCs), including B cells, dendritic cells, and macrophages, CD86 is the ligand for two proteins at the cell surface of T cells, CD28 antigen and cytotoxic T-lymphocyte-associated protein 4. Binding of CD86 with CD28 antigen is a costimulatory signal for activation of the T-cell. Binding of CD86 with cytotoxic T-lymphocyte-associated protein 4 negatively regulates T-cell activation and diminishes the immune response.