Anti-Human CD86 (BU63) Mouse IgG2a Recombinant Antibody, PBS Only

CD86 Uni-rAb^TM Recombinant Antibody for FC

Host / Isotype

Mouse / IgG2a

Reactivity

human

Applications

FC

Conjugate

Unconjugated

CloneNo.

BU63

Cat no : 65592-1-PBS

Synonyms

CD28LG2, BU63, B70, B7 2, Activation B7-2 antigen

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Validation Data Gallery

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  FC experiment of human PBMCs using 65592-1-MR (same clone as 65592-1-PBS)

  1x10^6 human PBMCs were surface stained with 0.25 ug Anti-Human CD86 Mouse IgG2a Recombinant Antibody (65592-1-MR, Clone: BU63) or Mouse IgG2a Isotype Control (65208-1-Ig, Clone: C1.18.4) and CoraLite®488-Conjugated AffiniPure Donkey Anti-Mouse IgG(H+L). Cells were then stained with APC Anti-Human CD14. Cells were incubated with FC Receptor Block prior to staining. Cells were not fixed. This data was developed using the same antibody clone with 65592-1-PBS in a different storage buffer formulation.

• 

  FC experiment of human PBMCs using 65592-1-MR (same clone as 65592-1-PBS)

  1x10^6 human PBMCs were surface stained with 0.25 ug Anti-Human CD86 Mouse IgG2a Recombinant Antibody (65592-1-MR, Clone:BU63) (red) or Mouse IgG2a Isotype Control (65208-1-Ig, Clone: C1.18.4) (blue) and CoraLite®488-Conjugated AffiniPure Donkey Anti-Mouse IgG(H+L). Cells were incubated with FC Receptor Block prior to staining. Cells were not fixed. This data was developed using the same antibody clone with 65592-1-PBS in a different storage buffer formulation.

• 

  FC experiment of human PBMCs using 65592-1-MR (same clone as 65592-1-PBS)

  1x10^6 human PBMCs were surface stained with 0.25 ug Anti-Human CD86 Mouse IgG2a Recombinant Antibody (65592-1-MR, Clone: BU63) (left) or 0.25 ug Anti-Human CD86 (65165-1-Ig, Clone: BU63) (right) and CoraLite®488-Conjugated AffiniPure Donkey Anti-Mouse IgG(H+L). Cells were then stained with APC Anti-Human CD14. Cells were incubated with FC Receptor Block prior to staining. Cells were not fixed. This data was developed using the same antibody clone with 65592-1-PBS in a different storage buffer formulation.

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Product Information

65592-1-PBS targets CD86 in FC applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Mouse / IgG2a
• Class: Recombinant
• Type: Antibody
• Immunogen: B-lymphoblastoid cell line ARH 77
• Full Name: CD86 molecule
• Calculated Molecular Weight: 329 aa, 38 kDa
• GenBank Accession Number: BC040261
• Gene Symbol: CD86
• Gene ID (NCBI): 942
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

CD86 (also known as B7.2) is a costimulatory molecule belonging to the immunoglobulin superfamily. Primarily expressed on antigen-presenting cells (APCs), including B cells, dendritic cells, and macrophages, CD86 is the ligand for two proteins at the cell surface of T cells, CD28 antigen and cytotoxic T-lymphocyte-associated protein 4. Binding of CD86 with CD28 antigen is a costimulatory signal for activation of the T-cell. Binding of CD86 with cytotoxic T-lymphocyte-associated protein 4 negatively regulates T-cell activation and diminishes the immune response.