FC experiment of human PBMCs using 65514-1-RR (same clone as 65514-1-PBS)
1x10^6 human PBMCs were treated with FC Receptor Block then surface stained with 0.1 ug Anti-Human CD86 Rabbit Recombinant Antibody (65514-1-RR, Clone:BU63) or Rabbit IgG control PolyAb (30000-0-AP), and CoraLite®647-conjugated AffiniPure F(ab')2 Fragment Donkey Anti-Rabbit IgG (H+L) (SA00014-7). Cells were not fixed. Monocytes were gated. This data was developed using the same antibody clone with 65514-1-PBS in a different storage buffer formulation.
1x10^6 human PBMCs were treated with FC Receptor Block then surface stained with 0.1 ug Anti-Human CD86 Rabbit Recombinant Antibody (65514-1-RR, Clone:BU63) or Rabbit IgG control PolyAb (30000-0-AP), and CoraLite®647-conjugated AffiniPure F(ab')2 Fragment Donkey Anti-Rabbit IgG (H+L) (SA00014-7). Cells were not fixed. Monocytes were gated. This data was developed using the same antibody clone with 65514-1-PBS in a different storage buffer formulation.
FC experiment of human PBMCs using 65514-1-PBS
1x10^6 human PBMCs were treated with FC Receptor Block then surface stained with 0.1 ug Anti-Human CD86 Rabbit Recombinant Antibody (65514-1-RR, Clone:BU63) or Rabbit IgG control PolyAb (30000-0-AP), and CoraLite®647-conjugated AffiniPure F(ab')2 Fragment Donkey Anti-Rabbit IgG (H+L) (SA00014-7). Cells were not fixed. Monocytes were gated.
1x10^6 human PBMCs were treated with FC Receptor Block then surface stained with 0.1 ug Anti-Human CD86 Rabbit Recombinant Antibody (65514-1-RR, Clone:BU63) or Rabbit IgG control PolyAb (30000-0-AP), and CoraLite®647-conjugated AffiniPure F(ab')2 Fragment Donkey Anti-Rabbit IgG (H+L) (SA00014-7). Cells were not fixed. Monocytes were gated.
FC experiment of human PBMCs using 65514-1-PBS
1x10^6 human PBMCs were treated with FC Receptor Block then surface stained with 0.1 ug Anti-Human CD86 Rabbit Recombinant Antibody (65514-1-RR, Clone:BU63) or Rabbit IgG control PolyAb (30000-0-AP), and CoraLite®647-conjugated AffiniPure F(ab')2 Fragment Donkey Anti-Rabbit IgG (H+L) (SA00014-7). Cells were not fixed. Monocytes were gated.
1x10^6 human PBMCs were treated with FC Receptor Block then surface stained with 0.1 ug Anti-Human CD86 Rabbit Recombinant Antibody (65514-1-RR, Clone:BU63) or Rabbit IgG control PolyAb (30000-0-AP), and CoraLite®647-conjugated AffiniPure F(ab')2 Fragment Donkey Anti-Rabbit IgG (H+L) (SA00014-7). Cells were not fixed. Monocytes were gated.
FC experiment of human PBMCs using 65514-1-RR (same clone as 65514-1-PBS)
1x10^6 human PBMCs were treated with FC Receptor Block then surface stained with 0.1 ug Anti-Human CD86 Rabbit Recombinant Antibody (65514-1-RR, Clone:BU63) or Rabbit IgG control PolyAb (30000-0-AP), and CoraLite®647-conjugated AffiniPure F(ab')2 Fragment Donkey Anti-Rabbit IgG (H+L) (SA00014-7). Cells were not fixed. Monocytes were gated. This data was developed using the same antibody clone with 65514-1-PBS in a different storage buffer formulation.
1x10^6 human PBMCs were treated with FC Receptor Block then surface stained with 0.1 ug Anti-Human CD86 Rabbit Recombinant Antibody (65514-1-RR, Clone:BU63) or Rabbit IgG control PolyAb (30000-0-AP), and CoraLite®647-conjugated AffiniPure F(ab')2 Fragment Donkey Anti-Rabbit IgG (H+L) (SA00014-7). Cells were not fixed. Monocytes were gated. This data was developed using the same antibody clone with 65514-1-PBS in a different storage buffer formulation.
FC experiment of human PBMCs using 65514-1-RR (same clone as 65514-1-PBS)
1x10^6 human PBMCs were treated with FC Receptor Block then surface stained with 0.1 ug Anti-Human CD86 Rabbit Recombinant Antibody (65514-1-RR, Clone:BU63) and CoraLite®647-conjugated AffiniPure F(ab')2 Fragment Donkey Anti-Rabbit IgG (H+L) (SA00014-7) (left and red), or stained with 0.1 ug Anti-Human CD86 Mouse Monoclonal Antibody (Clone:BU63) and CoraLite®647-conjugated AffiniPure F(ab')2 Fragment Donkey Anti-Mouse IgG (H+L) (SA00014-8). Cells were not fixed. Monocytes were gated. This data was developed using the same antibody clone with 65514-1-PBS in a different storage buffer formulation.
1x10^6 human PBMCs were treated with FC Receptor Block then surface stained with 0.1 ug Anti-Human CD86 Rabbit Recombinant Antibody (65514-1-RR, Clone:BU63) and CoraLite®647-conjugated AffiniPure F(ab')2 Fragment Donkey Anti-Rabbit IgG (H+L) (SA00014-7) (left and red), or stained with 0.1 ug Anti-Human CD86 Mouse Monoclonal Antibody (Clone:BU63) and CoraLite®647-conjugated AffiniPure F(ab')2 Fragment Donkey Anti-Mouse IgG (H+L) (SA00014-8). Cells were not fixed. Monocytes were gated. This data was developed using the same antibody clone with 65514-1-PBS in a different storage buffer formulation.
FC experiment of human PBMCs using 65514-1-PBS
1x10^6 human PBMCs were treated with FC Receptor Block then surface stained with 0.1 ug Anti-Human CD86 Rabbit Recombinant Antibody (65514-1-RR, Clone:BU63) and CoraLite®647-conjugated AffiniPure F(ab')2 Fragment Donkey Anti-Rabbit IgG (H+L) (SA00014-7) (left and red), or stained with 0.1 ug Anti-Human CD86 Mouse Monoclonal Antibody (Clone:BU63) and CoraLite®647-conjugated AffiniPure F(ab')2 Fragment Donkey Anti-Mouse IgG (H+L) (SA00014-8). Cells were not fixed. Monocytes were gated.
1x10^6 human PBMCs were treated with FC Receptor Block then surface stained with 0.1 ug Anti-Human CD86 Rabbit Recombinant Antibody (65514-1-RR, Clone:BU63) and CoraLite®647-conjugated AffiniPure F(ab')2 Fragment Donkey Anti-Rabbit IgG (H+L) (SA00014-7) (left and red), or stained with 0.1 ug Anti-Human CD86 Mouse Monoclonal Antibody (Clone:BU63) and CoraLite®647-conjugated AffiniPure F(ab')2 Fragment Donkey Anti-Mouse IgG (H+L) (SA00014-8). Cells were not fixed. Monocytes were gated.
The Proteintech guarantee covers Proteintech antibodies in any species and any application, including those not listed on the datasheet. If the antibody doesn’t perform, you can receive a hassle-free refund or credit note.
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Product Information
65514-1-PBS targets CD86 in FC applications and shows reactivity with human samples.
Store at -80°C. Stable for one year after shipment.
Background Information
CD86 (also known as B7-2) is a costimulatory molecule belonging to the immunoglobulin superfamily. Primarily expressed on antigen-presenting cells (APCs), including B cells, dendritic cells, and macrophages, CD86 is the ligand for two proteins at the cell surface of T cells, CD28 antigen and CTLA-4. Binding of CD86 with CD28 antigen is a costimulatory signal for activation of the T-cell. Binding of CD86 with CTLA-4 negatively regulates T-cell activation and diminishes the immune response. (PMID: 7513726; 1847722; 11029388; 27591335)
FC experiment of human PBMCs using 65514-1-RR (same clone as 65514-1-PBS)
1x10^6 human PBMCs were treated with FC Receptor Block then surface stained with 0.1 ug Anti-Human CD86 Rabbit Recombinant Antibody (65514-1-RR, Clone:BU63) or Rabbit IgG control PolyAb (30000-0-AP), and CoraLite®647-conjugated AffiniPure F(ab')2 Fragment Donkey Anti-Rabbit IgG (H+L) (SA00014-7). Cells were not fixed. Monocytes were gated. This data was developed using the same antibody clone with 65514-1-PBS in a different storage buffer formulation.
FC experiment of human PBMCs using 65514-1-PBS
1x10^6 human PBMCs were treated with FC Receptor Block then surface stained with 0.1 ug Anti-Human CD86 Rabbit Recombinant Antibody (65514-1-RR, Clone:BU63) or Rabbit IgG control PolyAb (30000-0-AP), and CoraLite®647-conjugated AffiniPure F(ab')2 Fragment Donkey Anti-Rabbit IgG (H+L) (SA00014-7). Cells were not fixed. Monocytes were gated.
FC experiment of human PBMCs using 65514-1-PBS
1x10^6 human PBMCs were treated with FC Receptor Block then surface stained with 0.1 ug Anti-Human CD86 Rabbit Recombinant Antibody (65514-1-RR, Clone:BU63) or Rabbit IgG control PolyAb (30000-0-AP), and CoraLite®647-conjugated AffiniPure F(ab')2 Fragment Donkey Anti-Rabbit IgG (H+L) (SA00014-7). Cells were not fixed. Monocytes were gated.
FC experiment of human PBMCs using 65514-1-RR (same clone as 65514-1-PBS)
1x10^6 human PBMCs were treated with FC Receptor Block then surface stained with 0.1 ug Anti-Human CD86 Rabbit Recombinant Antibody (65514-1-RR, Clone:BU63) or Rabbit IgG control PolyAb (30000-0-AP), and CoraLite®647-conjugated AffiniPure F(ab')2 Fragment Donkey Anti-Rabbit IgG (H+L) (SA00014-7). Cells were not fixed. Monocytes were gated. This data was developed using the same antibody clone with 65514-1-PBS in a different storage buffer formulation.
FC experiment of human PBMCs using 65514-1-RR (same clone as 65514-1-PBS)
1x10^6 human PBMCs were treated with FC Receptor Block then surface stained with 0.1 ug Anti-Human CD86 Rabbit Recombinant Antibody (65514-1-RR, Clone:BU63) and CoraLite®647-conjugated AffiniPure F(ab')2 Fragment Donkey Anti-Rabbit IgG (H+L) (SA00014-7) (left and red), or stained with 0.1 ug Anti-Human CD86 Mouse Monoclonal Antibody (Clone:BU63) and CoraLite®647-conjugated AffiniPure F(ab')2 Fragment Donkey Anti-Mouse IgG (H+L) (SA00014-8). Cells were not fixed. Monocytes were gated. This data was developed using the same antibody clone with 65514-1-PBS in a different storage buffer formulation.
FC experiment of human PBMCs using 65514-1-PBS
1x10^6 human PBMCs were treated with FC Receptor Block then surface stained with 0.1 ug Anti-Human CD86 Rabbit Recombinant Antibody (65514-1-RR, Clone:BU63) and CoraLite®647-conjugated AffiniPure F(ab')2 Fragment Donkey Anti-Rabbit IgG (H+L) (SA00014-7) (left and red), or stained with 0.1 ug Anti-Human CD86 Mouse Monoclonal Antibody (Clone:BU63) and CoraLite®647-conjugated AffiniPure F(ab')2 Fragment Donkey Anti-Mouse IgG (H+L) (SA00014-8). Cells were not fixed. Monocytes were gated.
The species listed in Tested Reactivity are in-house verified and applicable species. For unlisted species, please refer to the homology analysis of the immunogen sequence and related species. For rabbit polyclonal antibodies, homology >70% is recommended. For mouse monoclonal antibodies and rabbit recombinant antibodies, homology >90% is recommended. Generally, the higher the homology, the greater the applicability. However, there will be certain differences in protein expression in different species, tissues or cells. Therefore, the homology analysis results are for reference only and do not serve as a guarantee.
At Proteintech, we pride ourselves on our antibody quality, customer service and transparency. As such, we are comparing our antibodies with other vendors, enabling easy identification and comparisons of key data to help you choose the suitable antibody for your needs.
We have selected the top cited antibodies from these vendors for you to compare.
Proteintech
CD86 Recombinant antibody
Catalog Number
65514-1-PBS
Citations
-
Dilutions
Applications
FC
Reactivity
human
Product Guarantee
Covers any species including not listed on datasheet
Covers any applications including not listed on datasheet
