Anti-Rat CD86 (24F)

CD86 Monoclonal Antibody for FC

Host / Isotype

Mouse / IgG1, kappa

Reactivity

rat

Applications

IF, FC

Conjugate

Unconjugated

Publications

1

CloneNo.

24F

Cat no : 65224-1-Ig

Synonyms

CD86 molecule, B7-2, B7 2, 24F

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Validation Data Gallery

• 

  FC experiment of Wistar Rat splenocytes using 65224-1-Ig

  1X10^6 unstimulated or LPS-stimulated Wistar rat splenocytes were surface stained with 0.5 ug Anti-Rat CD86 (65224-1-Ig, Clone:24F) and FITC anti-mouse IgG1 Antibody at dilution 1:100. Cells were not fixed.

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Tested Applications

• Positive FC detected in: LPS-stimulated Wistar rat splenocytes

Recommended dilution

Application	Dilution
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

• IF: See 1 publications below
• FC: See 1 publications below

Product Information

65224-1-Ig targets CD86 in IF, FC applications and shows reactivity with rat samples.

• Tested Reactivity: rat
• Cited Reactivity: rat
• Host / Isotype: Mouse / IgG1, kappa
• Class: Monoclonal
• Type: Antibody
• Immunogen: HTLV-1-transformed Lewis-S1 rat T cell line
• Full Name: CD86 molecule
• Calculated Molecular Weight: 36 kDa
• GenBank Accession Number: NM_020081
• Gene Symbol: Cd86
• Gene ID (NCBI): 56822
• RRID: AB_3670278
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Affinity purification
• Storage Buffer: PBS with 0.09% sodium azide.
• Storage Conditions: Store at 2-8°C. Stable for one year after shipment.

Background Information

CD86 (also known as B7.2) is a costimulatory molecule belonging to the immunoglobulin superfamily. Primarily expressed on antigen-presenting cells (APCs), including B cells, dendritic cells, and macrophages, CD86 is the ligand for two proteins at the cell surface of T cells, CD28 antigen and cytotoxic T-lymphocyte-associated protein 4. Binding of CD86 with CD28 antigen is a costimulatory signal for activation of the T-cell. Binding of CD86 with cytotoxic T-lymphocyte-associated protein 4 negatively regulates T-cell activation and diminishes the immune response.

Publications

Species	Application	Title
rat	IF,FC	Prostaglandins Other Lipid Mediat; Protective role of Yougui Yin in experimental knee osteoarthritis: from the perspective of macrophage polarization; Authors - Zhongqing Wu