CD86 (C-terminal) Polyclonal antibody

CD86 (C-terminal) Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse and More (1)

Applications

WB, IHC, IF, ELISA

Conjugate

Unconjugated

Publications

24

Cat no : 26903-1-AP

Synonyms

Activation B7 2 antigen, B7 2, B70, CD28LG2, CD86, CD86 (C-terminal), CD86 molecule, CTLA 4 counter receptor B7.2, FUN 1, LAB72

---

Validation Data Gallery

• 

  WB analysis using 26903-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 26903-1-AP (CD86 (C-terminal) antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 26903-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 26903-1-AP (CD86 (C-terminal) antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

---

Tested Applications

• Positive WB detected in: Daudi cells, Raji cell, Ramos cells, RAW 264.7 cells, LPS-treated RAW 264.7 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:3000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 12 publications below
• IHC: See 5 publications below
• IF: See 12 publications below

Product Information

26903-1-AP targets CD86 (C-terminal) in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Cited Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: CD86 (C-terminal) fusion protein Ag25432
• Full Name: CD86 molecule
• Calculated Molecular Weight: 329 aa, 38 kDa
• Observed Molecular Weight: 65 kDa
• GenBank Accession Number: BC040261
• Gene Symbol: CD86
• Gene ID (NCBI): 942
• RRID: AB_2880677
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

CD86 (also known as B7.2) is a costimulatory molecule belonging to the immunoglobulin superfamily. Primarily expressed on antigen-presenting cells (APCs), including B cells, dendritic cells, and macrophages, CD86 is the ligand for two proteins at the cell surface of T cells, CD28 antigen and cytotoxic T-lymphocyte-associated protein 4. Binding of CD86 with CD28 antigen is a costimulatory signal for activation of the T-cell. Binding of CD86 with cytotoxic T-lymphocyte-associated protein 4 negatively regulates T-cell activation and diminishes the immune response.

Publications

Species	Application	Title
mouse	WB	ACS Nano; Antigen Self-Presented Personalized Nanovaccines Boost the Immunotherapy of Highly Invasive and Metastatic Tumors; Authors - Tingting Wang
mouse	WB	Nat Commun; A photo-triggered self-accelerated nanoplatform for multifunctional image-guided combination cancer immunotherapy; Authors - Xiaoying Kang
mouse	IF	Theranostics; Multifunctionally disordered TiO2 nanoneedles prevent periprosthetic infection and enhance osteointegration by killing bacteria and modulating the osteoimmune microenvironment; Authors - Yangmengfan Chen
rat	WB,IF	Int J Biol Macromol; A multifunctional polydopamine/genipin/alendronate nanoparticle licences fibrin hydrogels osteoinductive and immunomodulatory potencies for repairing bone defects; Authors - Wentao Shi
mouse	IHC,IF	Int Immunopharmacol; Tryptanthrin promotes pressure ulcers healing in mice by inhibiting macrophage-mediated inflammation via cGAS/STING pathways; Authors - Yaozhi He
human	WB	Cancer Immunol Immunother; Novel roles of VAT1 expression in the immunosuppressive action of diffuse gliomas.; Authors - Pei Yang