CD86 Polyclonal antibody

CD86 Polyclonal Antibody for WB, IF, FC, ELISA

Host / Isotype

Rabbit / IgG


human, mouse and More (1)


WB, IF, FC, IHC, ELISA, Cell treatment



Cat no : 13395-1-AP


Activation B7 2 antigen, B7 2, B70, CD28LG2, CD86, CD86 molecule, FUN 1, LAB72

Tested Applications

Positive WB detected inDaudi cells, Raji cells, Ramos cells, RAW 264.7 cells
Positive IF detected inraji cells
Positive FC detected inRaji cells

Recommended dilution

Western Blot (WB)WB : 1:1000-1:4000
Immunofluorescence (IF)IF : 1:50-1:500
Flow Cytometry (FC)FC : 0.20 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

13395-1-AP targets CD86 in WB, IF, FC, IHC, ELISA, Cell treatment applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Cited Reactivityhuman, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen CD86 fusion protein Ag4063
Full Name CD86 molecule
Calculated Molecular Weight 329 aa, 38 kDa
Observed Molecular Weight 60-80 kDa
GenBank Accession NumberBC040261
Gene Symbol CD86
Gene ID (NCBI) 942
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

CD86 (also known as B7.2) is a costimulatory molecule belonging to the immunoglobulin superfamily. Primarily expressed on antigen-presenting cells (APCs), including B cells, dendritic cells, and macrophages, CD86 is the ligand for two proteins at the cell surface of T cells, CD28 antigen and cytotoxic T-lymphocyte-associated protein 4. Binding of CD86 with CD28 antigen is a costimulatory signal for activation of the T-cell. Binding of CD86 with cytotoxic T-lymphocyte-associated protein 4 negatively regulates T-cell activation and diminishes the immune response. This antibody detects a band of approximately 65-70 kDa, consistent with glycosylated form of CD86 (PMID: 8592066, 21849678).


Product Specific Protocols
WB protocol for CD86 antibody 13395-1-APDownload protocol
IF protocol for CD86 antibody 13395-1-APDownload protocol
FC protocol for CD86 antibody 13395-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols



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The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


Martin (Verified Customer) (09-28-2022)

Antibody is working very well

  • Applications: Immunohistochemistry
  • Primary Antibody Dilution: 1:300