1x10^6 human PBMCs were surface stained with 0.25 ug Anti-Human CD85j / LILRB1 (GHI/75) Mouse IgG2a Recombinant Antibody (65623-1-MR, Clone: GHI/75) or Mouse IgG2a Isotype Control (C1.18.4) (65208-1-Ig, Clone: C1.18.4) and CoraLite®488-Conjugated Donkey Anti-Mouse IgG(H+L). Cells were incubated with FC Receptor Block prior to staining. Cells were not fixed.
1x10^6 human PBMCs were surface stained with 0.25 ug Anti-Human CD85j / LILRB1 (GHI/75) Mouse IgG2a Recombinant Antibody (65623-1-MR, Clone: GHI/75) or Mouse IgG2a Isotype Control (C1.18.4) (65208-1-Ig, Clone: C1.18.4) and CoraLite®488-Conjugated Donkey Anti-Mouse IgG(H+L). Cells were incubated with FC Receptor Block prior to staining. Cells were not fixed.
FC experiment of human PBMCs using 65623-1-MR
1x10^6 human PBMCs were surface stained with 0.25 ug Anti-Human CD85j / LILRB1 (GHI/75) Mouse IgG2a Recombinant Antibody (65623-1-MR, Clone: GHI/75) (red) or Mouse IgG2a Isotype Control (C1.18.4) (65208-1-Ig, Clone: C1.18.4) (blue) and CoraLite®488-Conjugated Donkey Anti-Mouse IgG(H+L). Cells were incubated with FC Receptor Block prior to staining. Cells were not fixed.
1x10^6 human PBMCs were surface stained with 0.25 ug Anti-Human CD85j / LILRB1 (GHI/75) Mouse IgG2a Recombinant Antibody (65623-1-MR, Clone: GHI/75) (red) or Mouse IgG2a Isotype Control (C1.18.4) (65208-1-Ig, Clone: C1.18.4) (blue) and CoraLite®488-Conjugated Donkey Anti-Mouse IgG(H+L). Cells were incubated with FC Receptor Block prior to staining. Cells were not fixed.
The Proteintech guarantee covers Proteintech antibodies in any species and any application, including those not listed on the datasheet. If the antibody doesn’t perform, you can receive a hassle-free refund or credit note.
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.
Product Information
65623-1-MR targets CD85j / LILRB1 in FC applications and shows reactivity with human samples.
Store at 2-8°C. Stable for one year after shipment.
Background Information
LILRB1, also named as CD85j or ILT2, includes four C2 Ig domains binding HLA class I molecules in the extracellular region and a cytoplasmic domain containing four immunoreceptor tyrosine-based inhibition motifs (ITIM). LILRB1 is a surface glycoprotein detected on the surface of B cells, monocytes, dendritic cells, T cells and NK cells.
Protocols
Product Specific Protocols
FC protocol for CD85j / LILRB1 antibody 65623-1-MR
1x10^6 human PBMCs were surface stained with 0.25 ug Anti-Human CD85j / LILRB1 (GHI/75) Mouse IgG2a Recombinant Antibody (65623-1-MR, Clone: GHI/75) or Mouse IgG2a Isotype Control (C1.18.4) (65208-1-Ig, Clone: C1.18.4) and CoraLite®488-Conjugated Donkey Anti-Mouse IgG(H+L). Cells were incubated with FC Receptor Block prior to staining. Cells were not fixed.
FC experiment of human PBMCs using 65623-1-MR
1x10^6 human PBMCs were surface stained with 0.25 ug Anti-Human CD85j / LILRB1 (GHI/75) Mouse IgG2a Recombinant Antibody (65623-1-MR, Clone: GHI/75) (red) or Mouse IgG2a Isotype Control (C1.18.4) (65208-1-Ig, Clone: C1.18.4) (blue) and CoraLite®488-Conjugated Donkey Anti-Mouse IgG(H+L). Cells were incubated with FC Receptor Block prior to staining. Cells were not fixed.
The species listed in Tested Reactivity are in-house verified and applicable species. For unlisted species, please refer to the homology analysis of the immunogen sequence and related species. For rabbit polyclonal antibodies, homology >70% is recommended. For mouse monoclonal antibodies and rabbit recombinant antibodies, homology >90% is recommended. Generally, the higher the homology, the greater the applicability. However, there will be certain differences in protein expression in different species, tissues or cells. Therefore, the homology analysis results are for reference only and do not serve as a guarantee.
At Proteintech, we pride ourselves on our antibody quality, customer service and transparency. As such, we are comparing our antibodies with other vendors, enabling easy identification and comparisons of key data to help you choose the suitable antibody for your needs.
We have selected the top cited antibodies from these vendors for you to compare.
Proteintech
CD85j / LILRB1 Recombinant antibody
Catalog Number
65623-1-MR
Citations
-
Dilutions
Applications
FC
Reactivity
human
Product Guarantee
Covers any species including not listed on datasheet
Covers any applications including not listed on datasheet
