CD83 Recombinant antibody, PBS Only (Detector)

CD83 Uni-rAb^TM Recombinant Antibody for WB, Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test

Conjugate

Unconjugated

CloneNo.

241855G5

Cat no : 84796-3-PBS

Synonyms

CD83 molecule, CD83 antigen, BL11, B-cell activation protein, B cell activation protein

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Validation Data Gallery

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  WB analysis using 84796-3-RR (same clone as 84796-3-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 84796-3-RR (CD83 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84796-3-PBS in a different storage buffer formulation.

• 

  Sandwich ELISA standard curve of MP01570-3

  Sandwich ELISA standard curve of MP01570-3, Human CD83 Recombinant Matched Antibody Pair - PBS only. 84796-4-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard Eg2033. 84796-3-PBS was HRP conjugated as the detection antibody. Range: 19.5-1250 pg/mL

• 

  Sample test of MP01570-3

  Serum of six individual healthy human donors was measured. The human CD83 concentration of detected samples was determined to be 372.6 pg/mL with a range of 167.8 - 934.1 pg/mL.

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  Cytometric bead array standard curve of MP01570-1

  Cytometric bead array standard curve of MP01570-1, CD83 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 84796-1-PBS. Detection antibody: 84796-3-PBS. Standard: Eg2033. Range: 0.313-40 ng/mL.

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Product Information

84796-3-PBS targets CD83 as part of a matched antibody pair:

MP01570-1: 84796-1-PBS capture and 84796-3-PBS detection (validated in Cytometric bead array)

MP01570-3: 84796-4-PBS capture and 84796-3-PBS detection (validated in Sandwich ELISA)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: Recombinant protein
• Full Name: CD83 molecule
• Calculated Molecular Weight: 23 kDa
• Observed Molecular Weight: 40-45 kDa
• GenBank Accession Number: NM_004233.4
• Gene Symbol: CD83
• Gene ID (NCBI): 9308
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

CD83 is a member of the immunoglobulin (Ig) superfamily, consisting of an extracellular V-type Ig-like domain, a transmembrane domain, and a cytoplasmic tail. CD83 is one of the most prominent markers for fully matured dendritic cells (DCs) (PMID: 17334966). It is also found on the surface of other activated hematopoietic cells, including lymphocytes, monocytes, macrophages, and neutrophils (PMID: 31231400; 17334966). CD83 regulates the maturation, activation, and homeostasis of numerous immune cells (PMID: 31231400; 32362900). CD83 can also expressed as a soluble form. Soluble CD83 can bind to DCs and inhibit their maturation (PMID: 17334966; 12403928).