CoraLite®594-conjugated CD82 Monoclonal antibody

CD82 Monoclonal Antibody for IF-P

Host / Isotype

Mouse / IgG2a

Reactivity

human, mouse

Applications

IF-P

Conjugate

CoraLite®594 Fluorescent Dye

CloneNo.

1E7A4

Cat no : CL594-66803

Synonyms

CD82, KAI1, Metastasis suppressor Kangai-1, IA4, CD82,KAI1, CD 82

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Validation Data Gallery

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  IF Staining of human tonsillitis using CL594-66803

  Immunofluorescent analysis of (4% PFA) fixed human tonsillitis tissue using CoraLite®594 CD82 antibody (CL594-66803, Clone: 1E7A4 ) at dilution of 1:200.

• 

  IF Staining of human tonsillitis using CL594-66803

  Immunofluorescent analysis of (4% PFA) fixed human tonsillitis tissue using CoraLite®594 CD82 antibody (CL594-66803, Clone: 1E7A4 ) at dilution of 1:200.

• 

  IF Staining of human ovary tumor using CL594-66803

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded human ovary tumor tissue using CoraLite®594 CD82 antibody (CL594-66803, Clone: 1E7A4 ) at dilution of 1:200. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of human ovary tumor using CL594-66803

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded human ovary tumor tissue using CoraLite®594 CD82 antibody (CL594-66803, Clone: 1E7A4 ) at dilution of 1:200. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

• Positive IF-P detected in: human tonsillitis tissue, human ovary tumor tissue

Recommended dilution

• Immunofluorescence (IF)-P: IF-P : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

CL594-66803 targets CD82 in IF-P applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Host / Isotype: Mouse / IgG2a
• Class: Monoclonal
• Type: Antibody
• Immunogen: CD82 fusion protein Ag17513
• Full Name: CD82 molecule
• Calculated Molecular Weight: 30 kDa
• GenBank Accession Number: BC000726
• Gene Symbol: CD82
• Gene ID (NCBI): 3732
• RRID: AB_2920042
• Conjugate: CoraLite®594 Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 588 nm / 604 nm
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
• Storage Conditions: Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

CD82 is a membrane glycoprotein and belongs to the tetraspanin superfamily, many of which are implicated in the regulation of cell motility, morphology, fusion, signaling, fertilization, and differentiation. CD82 was originally identified as a suppressor of metastasis located on human chromosome 11p11.2 in prostate carcinoma. The majority of evidence indicates that CD82 expression is downregulated or abolished in a variety of malignant tumors. CD82 is present at high levels in human monocyte and macrophage lineages and in various epithelial cells in the prostate, lung, pancreas and many other tissues. In epithelial cells, CD82 is implicated in diverse biological processes such as cell adhesion, migration, apoptosis and morphogenesis.