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CD82 Recombinant antibody, PBS Only (Capture)

CD82 Uni-rAbTM Recombinant Antibody for IF/ICC, Cytometric bead array, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

IF/ICC, Cytometric bead array, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

241765E10

Cat no : 84617-3-PBS

Synonyms

CD82, KAI1, Metastasis suppressor Kangai-1, IA4, CD82,KAI1, CD 82

Validation Data Gallery

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  • Immunofluorescence (IF) / fluorescent staining of A549 cells using CD82 Recombinant antibody (84617-3-RR)

    IF Staining of A549 using 84617-3-RR (same clone as 84617-3-PBS)

    Immunofluorescent analysis of (4% PFA) fixed A549 cells using CD82 antibody (84617-3-RR, Clone: 241765E10 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 84617-3-PBS in a different storage buffer formulation.

  • Cytometric bead array standard curve of MP01456-1

    Cytometric bead array standard curve of MP01456-1

    Cytometric bead array standard curve of MP01456-1, CD82 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 84617-3-PBS. Detection antibody: 84617-4-PBS. Standard: Eg1749. Range: 0.313-40 ng/mL.

Product Information

84617-3-PBS targets CD82 as part of a matched antibody pair:

MP01456-1: 84617-3-PBS capture and 84617-4-PBS detection (validated in Cytometric bead array)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen Recombinant protein
Full Name CD82 molecule
Calculated Molecular Weight30 kDa
GenBank Accession NumberNM_002231.4
Gene Symbol CD82
Gene ID (NCBI) 3732
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

CD82 is a membrane glycoprotein and belongs to the tetraspanin superfamily, many of which are implicated in the regulation of cell motility, morphology, fusion, signaling, fertilization, and differentiation. CD82 was originally identified as a suppressor of metastasis located on human chromosome 11p11.2 in prostate carcinoma. The majority of evidence indicates that CD82 expression is downregulated or abolished in a variety of malignant tumors. CD82 is present at high levels in human monocyte and macrophage lineages and in various epithelial cells in the prostate, lung, pancreas and many other tissues. In epithelial cells, CD82 is implicated in diverse biological processes such as cell adhesion, migration, apoptosis and morphogenesis.