CD82 Polyclonal antibody, PBS Only

CD82 Polyclonal Antibody for WB, IHC, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, Indirect ELISA

Conjugate

Unconjugated

Cat no : 10248-1-PBS

Synonyms

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Validation Data Gallery

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  WB analysis of K-562 using 10248-1-AP (same clone as 10248-1-PBS)

  K-562 cells were subjected to SDS PAGE followed by western blot with 10248-1-AP (CD82 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 10248-1-PBS in a different storage buffer formulation.

• 

  WB analysis of mouse brain using 10248-1-AP (same clone as 10248-1-PBS)

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 10248-1-AP (CD82 antibody) at dilution of 1:0 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 10248-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human tonsillitis using 10248-1-AP (same clone as 10248-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 10248-1-AP (CD82 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 10248-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human tonsillitis using 10248-1-AP (same clone as 10248-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 10248-1-AP (CD82 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 10248-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human thyroid cancer using 10248-1-AP (same clone as 10248-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human thyroid cancer tissue slide using 10248-1-AP (CD82 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 10248-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human thyroid cancer using 10248-1-AP (same clone as 10248-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human thyroid cancer tissue slide using 10248-1-AP (CD82 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 10248-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human breast cancer using 10248-1-AP (same clone as 10248-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 10248-1-AP (CD82 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 10248-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human breast cancer using 10248-1-AP (same clone as 10248-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 10248-1-AP (CD82 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 10248-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human thyroid cancer using 10248-1-AP (same clone as 10248-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human thyroid cancer tissue slide using 10248-1-AP (CD82 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 10248-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human thyroid cancer using 10248-1-AP (same clone as 10248-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human thyroid cancer tissue slide using 10248-1-AP (CD82 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 10248-1-PBS in a different storage buffer formulation.

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Product Information

10248-1-PBS targets CD82 in WB, IHC, Indirect ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: CD82 fusion protein Ag0314
• Full Name: CD82 molecule
• Calculated Molecular Weight: 30 kDa
• Observed Molecular Weight: 30-34 kDa
• GenBank Accession Number: BC000726
• Gene Symbol: CD82
• Gene ID (NCBI): 3732
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

CD82 is a membrane glycoprotein and belongs to the tetraspanin superfamily, many of which are implicated in the regulation of cell motility, morphology, fusion, signaling, fertilization, and differentiation. CD82 was originally identified as a suppressor of metastasis located on human chromosome 11p11.2 in prostate carcinoma. The majority of evidence indicates that CD82 expression is downregulated or abolished in a variety of malignant tumors. CD82 is present at high levels in human monocyte and macrophage lineages and in various epithelial cells in the prostate, lung, pancreas and many other tissues. In epithelial cells, CD82 is implicated in diverse biological processes such as cell adhesion, migration, apoptosis and morphogenesis.