Anti-Human CD81 (5A6) Mouse IgG2a Recombinant Antibody

CD81 Uni-rAb^TM Recombinant Antibody for FC

Host / Isotype

Mouse / IgG2a

Reactivity

human

Applications

FC

Conjugate

Unconjugated

CloneNo.

5A6

Cat no : 65644-1-MR

Synonyms

TAPA1, S5.7, CD81 molecule, CD81 antigen, 5A6

---

Validation Data Gallery

• 

  FC experiment of human peripheral blood leukocytes using 65644-1-MR

  1x10^6 human peripheral blood leukocytes were surface stained with 0.25 ug Anti-Human CD81 (5A6) Mouse IgG2a Recombinant Antibody (65644-1-MR, Clone: 5A6) or Mouse IgG2a Isotype Control (C1.18.4) (65208-1-Ig, Clone: C1.18.4) and PE-conjugated Goat Anti-Mouse IgG. Cells were not fixed.

• 

  FC experiment of human peripheral blood leukocytes using 65644-1-MR

  1x10^6 human peripheral blood leukocytes were surface stained with 0.25 ug Anti-Human CD81 (5A6) Mouse IgG2a Recombinant Antibody (65644-1-MR, Clone: 5A6) (red) or Mouse IgG2a Isotype Control (C1.18.4) (65208-1-Ig, Clone: C1.18.4) (blue) and PE-conjugated Goat Anti-Mouse IgG. Cells were not fixed.

---

Tested Applications

• Positive FC detected in: human peripheral blood leukocytes

Recommended dilution

Application	Dilution
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

65644-1-MR targets CD81 in FC applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Mouse / IgG2a
• Class: Recombinant
• Type: Antibody
• Immunogen: Human OCI-LY8 cell line
• Full Name: CD81 molecule
• Calculated Molecular Weight: 26 kDa
• GenBank Accession Number: BC002978
• Gene Symbol: CD81
• Gene ID (NCBI): 975
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS with 0.09% sodium azide.
• Storage Conditions: Store at 2-8°C. Stable for one year after shipment.

Background Information

CD81 (also known as TAPA1 or TSPAN28) is a membrane protein of the tetraspanin superfamily, which are characterized by the presence of four conserved transmembrane regions. Many of these members are expressed on leukocytes and have been implicated in signal transduction, cell-cell interactions, and cellular activation and development. CD81 is involved in signal transduction and cell adhesion in the immune system (PMID: 9597125). CD81 has also been identified as en essential receptor for HCV (hepatitis C virus) (PMID: 21428934).