Anti-Human CD80 (B7-1) (2D10.4)

CD80 (B7-1) Monoclonal Antibody for FC

Host / Isotype

Mouse / IgG1, kappa

Reactivity

Human

Applications

FC

Conjugate

Unconjugated

Publications

1

CloneNo.

2D10.4

Cat no : 65083-1-Ig

Synonyms

Activation B7 1 antigen, B7, B7 1, BB1, CD28LG, CD28LG1, CD80, CD80 (B7-1), CD80 molecule, CTLA 4 counter receptor B7.1, LAB7

---

Validation Data Gallery

• 

  FC experiment of Daudi using 65083-1-Ig

  1X10^6 Daudi cells were surface stained with 0.25 ug Anti-Human CD80 (B7-1) (65083-1-Ig, Clone: 2D10.4) and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (green), or stained with 0.25 ug isotype control and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (black). Cells were not fixed.

---

Tested Applications

• Positive FC detected in: Daudi cells

Recommended dilution

Application	Dilution
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

• FC: See 1 publications below

Product Information

65083-1-Ig targets CD80 (B7-1) in FC applications and shows reactivity with Human samples.

• Tested Reactivity: Human
• Host / Isotype: Mouse / IgG1, kappa
• Class: Monoclonal
• Type: Antibody
• Immunogen: N/A
• Full Name: CD80 molecule
• Calculated Molecular Weight: 33 kDa
• GenBank Accession Number: BC042665
• Gene Symbol: CD80
• Gene ID (NCBI): 941
• RRID: AB_2918386
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Affinity purification
• Storage Buffer: PBS with 0.1% sodium azide, pH 7.3.
• Storage Conditions: Store at 2-8°C. Stable for one year after shipment.

Background Information

CD80 (also known as B7-1) is a type I membrane protein that is a member of the immunoglobulin superfamily, with an extracellular immunoglobulin constant-like domain and a variable-like domain required for receptor binding. It is expressed on antigen-presenting cells (APCs), including B cells, dendritic cells, monocytes, and macrophages. CD80 is the receptor for the proteins CD28 and CTLA-4 found on the surface of T-cells. It is involved in the costimulatory signal essential for T-lymphocyte activation. T-cell proliferation and cytokine production is induced by the binding of CD28, binding to CTLA-4 has opposite effects and inhibits T-cell activation. CD80 also acts as a cellular attachment receptor for adenovirus subgroup B. (PMID: 7545666; 12015893; 16920215)

Publications

Species	Application	Title
human	FC	Proc Natl Acad Sci U S A; Development of a highly sensitive platform for protein-protein interaction detection and regulation of T cell function; Authors - Hideki Hayashi