CD8 Monoclonal antibody, PBS Only

CD8 Monoclonal Antibody for WB, IF, IHC, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human

Applications

WB, IF, IHC, ELISA

Conjugate

Unconjugated

CloneNo.

1G2B10

Cat no : 66868-1-PBS

Synonyms

CD8, CD8A, CD8a molecule, Leu2, MAL, p32

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Validation Data Gallery

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  WB analysis of MOLT-4 using 66868-1-Ig (same clone as 66868-1-PBS)

  MOLT-4 cells were subjected to SDS PAGE followed by western blot with 66868-1-Ig (CD8a antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66868-1-PBS in a different storage buffer formulation.

• 

  WB analysis of human peripheral blood leukocyte using 66868-1-Ig (same clone as 66868-1-PBS)

  Human peripheral blood leukocyte cells were subjected to SDS PAGE followed by western blot with 66868-1-Ig (CD8a antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66868-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human tonsillitis using 66868-1-Ig (same clone as 66868-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 66868-1-Ig (CD8a antibody) at dilution of 1:20000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66868-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human tonsillitis using 66868-1-Ig (same clone as 66868-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 66868-1-Ig (CD8a antibody) at dilution of 1:20000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66868-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human pancreas cancer using 66868-1-Ig (same clone as 66868-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 66868-1-Ig (CD8a antibody) at dilution of 1:10000 (under 40x lens) Heat mediated antigen retrieved with Sodium Citrate buffer (pH 6.0). This data was developed using the same antibody clone with 66868-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human appendicitis using 66868-1-Ig (same clone as 66868-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human appendicitis tissue slide using 66868-1-Ig (CD8a antibody) at dilution of 1:4000 (under 10x lens) Heat mediated antigen retrieved with Sodium Citrate buffer (pH 6.0). This data was developed using the same antibody clone with 66868-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human appendicitis using 66868-1-Ig (same clone as 66868-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human appendicitis tissue slide using 66868-1-Ig (CD8a antibody) at dilution of 1:4000 (under 40x lens) Heat mediated antigen retrieved with Sodium Citrate buffer (pH 6.0). This data was developed using the same antibody clone with 66868-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human pancreas cancer using 66868-1-Ig (same clone as 66868-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 66868-1-Ig (CD8a antibody) at dilution of 1:10000 (under 10x lens) Heat mediated antigen retrieved with Sodium Citrate buffer (pH 6.0). This data was developed using the same antibody clone with 66868-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human pancreas cancer using 66868-1-Ig (same clone as 66868-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 66868-1-Ig (CD8a antibody) at dilution of 1:10000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66868-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human pancreas cancer using 66868-1-Ig (same clone as 66868-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 66868-1-Ig (CD8a antibody) at dilution of 1:10000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66868-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human colon cancer using 66868-1-Ig (same clone as 66868-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 66868-1-Ig (CD8a antibody) at dilution of 1:10000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66868-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human colon cancer using 66868-1-Ig (same clone as 66868-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 66868-1-Ig (CD8a antibody) at dilution of 1:10000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66868-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human colon cancer using 66868-1-Ig (same clone as 66868-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 66868-1-Ig (CD8a antibody) at dilution of 1:10000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66868-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human colon cancer using 66868-1-Ig (same clone as 66868-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 66868-1-Ig (CD8a antibody) at dilution of 1:10000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66868-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human breast cancer using 66868-1-Ig (same clone as 66868-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66868-1-Ig (CD8a antibody) at dilution of 1:10000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66868-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human breast cancer using 66868-1-Ig (same clone as 66868-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66868-1-Ig (CD8a antibody) at dilution of 1:10000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66868-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human lung cancer using 66868-1-Ig (same clone as 66868-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 66868-1-Ig (CD8a antibody) at dilution of 1:10000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66868-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human lung cancer using 66868-1-Ig (same clone as 66868-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 66868-1-Ig (CD8a antibody) at dilution of 1:10000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66868-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human breast cancer using 66868-1-Ig (same clone as 66868-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66868-1-Ig (CD8a antibody) at dilution of 1:10000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66868-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human breast cancer using 66868-1-Ig (same clone as 66868-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66868-1-Ig (CD8a antibody) at dilution of 1:10000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66868-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human lung cancer using 66868-1-Ig (same clone as 66868-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 66868-1-Ig (CD8a antibody) at dilution of 1:10000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66868-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human lung cancer using 66868-1-Ig (same clone as 66868-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 66868-1-Ig (CD8a antibody) at dilution of 1:10000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66868-1-PBS in a different storage buffer formulation.

• 

  IF Staining of human tonsillitis using 66868-1-Ig (same clone as 66868-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed human tonsillitis tissue using CD8a antibody (66868-1-Ig, Clone: 1G2B10 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66868-1-PBS in a different storage buffer formulation.

• 

  IF Staining of human tonsillitis using 66868-1-Ig (same clone as 66868-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed human tonsillitis tissue using CD8a antibody (66868-1-Ig, Clone: 1G2B10 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66868-1-PBS in a different storage buffer formulation.

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Recommended dilution

Application	Dilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

66868-1-PBS targets CD8 in WB, IF, IHC, ELISA applications and shows reactivity with Human samples.

• Tested Reactivity: Human
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: CD8 fusion protein Ag11111
• Full Name: CD8a molecule
• Calculated Molecular Weight: 235 aa, 26 kDa
• Observed Molecular Weight: 32 kDa
• GenBank Accession Number: BC025715
• Gene Symbol: CD8A
• Gene ID (NCBI): 925
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS only
• Storage Conditions: Store at -80°C.

Background Information

CD8 is a transmembrane glycoprotein that is predominantly expressed on the surface of cytotoxic T cells, and can also be found on natural killer cells, cortical thymocytes, and dendritic cells. CD8 serves as a co-receptor for the T cell receptor (TCR). Both CD8 and TCR recognize antigens displayed by an antigen presenting cell (APC) in the context of class I MHC molecules. CD8 plays a role in T cell development and activation of mature T cells. This antibody is raised against 23-183 aa of human CD8a, the alpha chain of CD8 molecule.