Anti-Rat CD8 (OX-8) Mouse IgG2a Recombinant Antibody, PBS Only

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  FC experiment of rat splenocytes using 65577-1-MR (same clone as 65577-1-PBS)

  1x10^6 rat splenocytes were surface stained with 0.25 ug Anti-Rat CD8a Mouse IgG2a Recombinant Antibody (65577-1-MR, Clone: OX-8) or Mouse IgG2a Isotype Control (65208-1-Ig, Clone: C1.18.4) and FITC anti-Mouse IgG2a Antibody. Cells were then stained with APC Anti-Rat CD4. Cells were not fixed. This data was developed using the same antibody clone with 65577-1-PBS in a different storage buffer formulation.

• 

  FC experiment of rat splenocytes using 65577-1-MR (same clone as 65577-1-PBS)

  1x10^6 rat splenocytes were surface stained with 0.25 ug Anti-Rat CD8 Mouse IgG2a Recombinant Antibody (65577-1-MR, Clone:OX-8) (red) or Mouse IgG2a Isotype Control (65208-1-Ig, Clone: C1.18.4) (blue) and FITC anti-Mouse IgG2a Antibody. Cells were not fixed. This data was developed using the same antibody clone with 65577-1-PBS in a different storage buffer formulation.

• 

  FC experiment of rat splenocytes using 65577-1-MR (same clone as 65577-1-PBS)

  1x10^6 rat splenocytes were surface stained with 0.25 ug Anti-Rat CD8a Mouse IgG2a Recombinant Antibody (65577-1-MR, Clone:OX-8) and FITC anti-Mouse IgG2a Antibody (left), or 0.25 ug Anti-Rat CD8a (65180-1-Ig, Clone: OX-8) and FITC anti-Mouse IgG1 Antibody (right). Cells were then stained with APC Anti-Rat CD4. Cells were not fixed. This data was developed using the same antibody clone with 65577-1-PBS in a different storage buffer formulation.

CD8 Recombinant Antibody for FC

Cat No. 65577-1-PBS

Clone No.OX-8

Host / Isotype

Mouse / IgG2a

Reactivity

rat

Applications

FC

Synonyms

Cd8a, OX-8, CD8a molecule

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Product Information

65577-1-PBS targets CD8 in FC applications and shows reactivity with rat samples.

• Tested Reactivity: rat
• Host / Isotype: Mouse / IgG2a
• Class: Recombinant
• Type: Antibody
• Immunogen: N/A
• Full Name: CD8a molecule
• GenBank Accession Number: BC088126
• Gene Symbol: Cd8a
• Gene ID (NCBI): 24930
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

CD8 is a transmembrane glycoprotein composed of two disulfide-linked chains. It can be present as a homodimer of CD8α or as a heterodimer of CD8α and CD8β (PMID: 3264320; 8253791). CD8 is found on most thymocytes. The majority of class I-restricted T cells express mostly the CD8αβ heterodimer while CD8αα homodimers alone have been found on some gut intraepithelial T cells , on some T cell receptor (TCR) γδ T cells and on NK cells (PMID: 2111591; 1831127; 8420975). CD8 acts as a co-receptor that binds to MHC class-I and participates in cytotoxic T cell activation (PMID: 8499079). During T cell development, CD8 is required for positive selection of CD4-/CD8+ T cells (PMID: 1968084).