CD79b Recombinant antibody

CD79b Uni-rAb^TM Recombinant Antibody for WB, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IF/ICC, ELISA

Conjugate

Unconjugated

CloneNo.

241273B7

Cat no : 84234-5-RR

Synonyms

IGB, Ig beta, B-cell-specific glycoprotein B29, B-cell antigen receptor complex-associated protein beta chain, B29

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Validation Data Gallery

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  WB analysis using 84234-5-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 84234-5-RR (CD79b antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  IF Staining of Raji using 84234-5-RR

  Immunofluorescent analysis of (4% PFA) fixed Raji cells using CD79b antibody (84234-5-RR, Clone: 241273B7 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

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  Affinity and Kinetic Characterization of 84234-5-RR

  Biolayer interferometry (BLl) kinetic assays of 84234-5-RR against Human CD79b were performed. The affinity constant is 3.23 nM.

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Tested Applications

• Positive WB detected in: Raji cells, Ramos cells, Daudi cells
• Positive IF/ICC detected in: Raji cells

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

84234-5-RR targets CD79b in WB, IF/ICC, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: Recombinant Protein
• Full Name: CD79b molecule, immunoglobulin-associated beta
• Calculated Molecular Weight: 229 aa, 26 kDa
• Observed Molecular Weight: 32-36 kDa
• GenBank Accession Number: BC032651
• Gene Symbol: CD79B
• Gene ID (NCBI): 974
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purfication
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

CD79 molecule encompasses two transmembrane proteins, CD79a and CD79b, which form a disulfide-linked heterodimer and are members of the immunoglobulin (Ig) gene superfamily (PMID: 2033258; 1375264; 2304550). CD79b molecule (also known as B29, IGB, AGM6, and Igbeta) is expressed almost exclusively on B cells and B-cell neoplasms, influencing antigen internalization and increasing antigen presentation efficiency (PMID: 7552998). CD79b mutation could be a key biomarker for DLBCL disease progression (PMID: 36182550).