Anti-Human CD79a (HM57) Mouse IgG2a Recombinant Antibody, PBS Only

CD79a Uni-rAb^TM Recombinant Antibody for FC (Intra)

Host / Isotype

Mouse / IgG2a

Reactivity

human

Applications

FC (Intra)

Conjugate

Unconjugated

CloneNo.

HM57

Cat no : 65610-1-PBS

Synonyms

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Validation Data Gallery

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  FC experiment of human PBMCs using 65610-1-MR (same clone as 65610-1-PBS)

  1x10^6 human PBMCs were intracellularly stained with 0.25 ug Anti-Human CD79a Mouse IgG2a Recombinant Antibody (65610-1-MR, Clone: HM57) or Mouse IgG2a Isotype Control (65208-1-Ig, Clone: C1.18.4), and CoraLite®488-Conjugated AffiniPure Donkey Anti-Mouse IgG(H+L). Cells were then stained with CoraLite® Plus 647 Anti-Human CD19. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). Lymphocytes were gated. This data was developed using the same antibody clone with 65610-1-PBS in a different storage buffer formulation.

• 

  FC experiment of human PBMCs using 65610-1-MR (same clone as 65610-1-PBS)

  1x10^6 human PBMCs were intracellularly stained with 0.25 ug Anti-Human CD79a Mouse IgG2a Recombinant Antibody (65610-1-MR, Clone: HM57) (red) or Mouse IgG2a Isotype Control (65208-1-Ig, Clone: C1.18.4) (blue), and CoraLite®488-Conjugated AffiniPure Donkey Anti-Mouse IgG(H+L). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). Lymphocytes were gated. This data was developed using the same antibody clone with 65610-1-PBS in a different storage buffer formulation.

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Product Information

65610-1-PBS targets CD79a in FC (Intra) applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Mouse / IgG2a
• Class: Recombinant
• Type: Antibody
• Immunogen: Synthetic peptide corresponding to 202-216 amino acid sequence of human CD79a
• Full Name: CD79a molecule, immunoglobulin-associated alpha
• GenBank Accession Number: BC113731
• Gene Symbol: CD79A
• Gene ID (NCBI): 973
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

CD79a, also named as B-cell antigen receptor complex-associated protein alpha chain or MB-1 membrane glycoprotein, is a 226 amino acid protein, which contains one ITAM domain and one Ig-like C2-type (immunoglobulin-like) domain. CD79a is expressed in B cell and localizes in the cell membrane. CD79a is required in cooperation with CD79b for initiation of the signal transduction cascade activated by binding of antigen to the B-cell antigen receptor complex (BCR) which leads to internalization of the complex, trafficking to late endosomes and antigen presentation. CD79a is also required for BCR surface expression and for efficient differentiation of pro- and pre-B-cells.