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CD79a Recombinant antibody, PBS Only

CD79a Uni-rAbTM Recombinant Antibody for WB, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

1F24

Cat no : 82747-4-PBS

Synonyms

MB 1, Ig-alpha, IGA, Ig alpha, B-cell antigen receptor complex-associated protein alpha chain

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using CD79a Recombinant antibody (82747-4-RR)

    WB analysis using 82747-4-RR (same clone as 82747-4-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 82747-4-RR (CD79a antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 82747-4-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 82747-4-RR

    Affinity and Kinetic Characterization of 82747-4-RR

    Biolayer interferometry (BLl) kinetic assays of 82747-4-RR against Human CD79A were performed. The affinity constant is 4.12 nM.

Product Information

82747-4-PBS targets CD79a in WB, Indirect ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen CD79a fusion protein Ag17924
Full Name CD79a molecule, immunoglobulin-associated alpha
Calculated Molecular Weight 226 aa, 25 kDa
Observed Molecular Weight40 kDa
GenBank Accession NumberBC113733
Gene Symbol CD79A
Gene ID (NCBI) 973
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

CD79a, also named as B-cell antigen receptor complex-associated protein alpha chain or MB-1 membrane glycoprotein, is a 226 amino acid protein, which contains one ITAM domain and one Ig-like C2-type (immunoglobulin-like) domain. CD79A is expressed in B cells and localizes in the cell membrane. CD79A is required in cooperation with CD79B for initiation of the signal transduction cascade activated by binding of antigen to the B-cell antigen receptor complex (BCR) which leads to internalization of the complex, trafficking to late endosomes and antigen presentation. CD79A is also required for BCR surface expression and for efficient differentiation of pro- and pre-B-cells.