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CD72 Recombinant antibody, PBS Only

CD72 Uni-rAbTM Recombinant Antibody for WB, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

241301B3

Cat no : 84270-5-PBS

Synonyms

LYB2, Lyb 2, CD72b, CD72 molecule, B-cell differentiation antigen CD72

Validation Data Gallery

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  • Western Blot (WB) analysis of Raji cells using CD72 Recombinant antibody (84270-5-RR)

    WB analysis of Raji using 84270-5-RR (same clone as 84270-5-PBS)

    Raji cells were subjected to SDS PAGE followed by western blot with 84270-5-RR (CD72 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84270-5-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 84270-5-RR

    Affinity and Kinetic Characterization of 84270-5-RR

    Biolayer interferometry (BLl) kinetic assays of 84270-5-RR against Human CD72 were performed. The affinity constant is 59.3 pM.

Product Information

84270-5-PBS targets CD72 in WB, Indirect ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen Recombinant Protein
Full Name CD72 molecule
Calculated Molecular Weight 359 aa, 40 kDa
GenBank Accession NumberBC030227
Gene Symbol CD72
Gene ID (NCBI) 971
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

CD72 is a type II transmembrane glycoprotein of 39-45 kDa and is expressed as a disulphide-linked homodimer (PMID: 1711157). CD72 is a pan B-cell marker covering the full range of differentiation from the very early stages of B cells to mature surface Ig+ B cells (PMID: 1384316). It is expressed in all stages of B cell development except plasma cells. CD72 is also expressed by dendritic cells, tissue macrophages in the red pulp of the spleen and von Kupffer cells in the liver (PMID: 8561576; 1384316). CD72 is the ligand for CD5 (PMID: 1711157). CD72 is a negative regulator of BCR signaling (PMID: 9590210). Interaction of CD100 with CD72 strictly tunes the strength of BCR signals and is essential for maintaining immunological homeostasis as well as generating a proper immune response (PMID: 16113236).