CD71 Monoclonal antibody
CD71 Monoclonal Antibody for WB, IHC
Host / Isotype
Mouse / IgG1, kappa
Reactivity
Human, Mouse
Applications
WB, IHC
Conjugate
Unconjugated
CloneNo.
H68.4
Cat no : 65236-1-Ig
Synonyms
Validation Data Gallery
Tested Applications
Positive WB detected in | PC-3 cells, RAW 264.7 cells, HeLa cells, K-562 cells |
Positive IHC detected in | human placenta tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:1000-1:6000 |
Immunohistochemistry (IHC) | IHC : 1:200-1:800 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
Product Information
65236-1-Ig targets CD71 in WB, IHC applications and shows reactivity with Human, Mouse samples.
Tested Reactivity | Human, Mouse |
Host / Isotype | Mouse / IgG1, kappa |
Class | Monoclonal |
Type | Antibody |
Immunogen | Recombinant protein |
Full Name | transferrin receptor (p90, CD71) |
Calculated Molecular Weight | 85 kDa |
Observed Molecular Weight | 90 kDa |
GenBank Accession Number | BC001188 |
Gene Symbol | CD71 |
Gene ID (NCBI) | 7037 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Affinity purification |
Storage Buffer | PBS with 0.09% sodium azide. |
Storage Conditions | Store at 2-8°C. Stable for one year after shipment. 20ul sizes contain 0.1% BSA. |
Background Information
CD71, also known as transferrin receptor protein 1 (TfR1), is a transmembrane glycoprotein composed of two disulfide-linked monomers, each of 90 kDa molecular weight. Each monomer binds one holo-transferrin molecule creating an iron-Tf-TfR complex which enters the cell by endocytosis. CD71 is present on actively proliferating cells and is essential for iron transport into proliferating cells.
Protocols
Product Specific Protocols | |
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WB protocol for CD71 antibody 65236-1-Ig | Download protocol |
IHC protocol for CD71 antibody 65236-1-Ig | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |