CD7 Monoclonal antibody, PBS Only

CD7 Monoclonal Antibody for IF, IHC, Indirect ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse

Applications

IF, IHC, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

1G10D8

Cat no : 60209-1-PBS

Synonyms

CD7, CD7 molecule, GP40, LEU 9, T cell antigen CD7, T cell leukemia antigen, T cell surface antigen Leu 9, Tp40, TP41

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Validation Data Gallery

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  WB analysis of Jurkat using 60209-1-Ig (same clone as 60209-1-PBS)

  Jurkat cells were subjected to SDS PAGE followed by western blot with 60209-1-Ig (CD7 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60209-1-PBS in a different storage buffer formulation.

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  IHC staining of human appendicitis using 60209-1-Ig (same clone as 60209-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human appendicitis tissue slide using 60209-1-Ig (CD7 antibody) at dilution of 1:3200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60209-1-PBS in a different storage buffer formulation.

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  IHC staining of human appendicitis using 60209-1-Ig (same clone as 60209-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human appendicitis tissue slide using 60209-1-Ig (CD7 antibody) at dilution of 1:3200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60209-1-PBS in a different storage buffer formulation.

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  IHC staining of human tonsillitis using 60209-1-Ig (same clone as 60209-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 60209-1-Ig (CD7 antibody) at dilution of 1:1600 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60209-1-PBS in a different storage buffer formulation.

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  IHC staining of human lymphoma using 60209-1-Ig (same clone as 60209-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human lymphoma using 60209-1-Ig(CD7 antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 60209-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human lymphoma using 60209-1-Ig (same clone as 60209-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human lymphoma using 60209-1-Ig(CD7 antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 60209-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse thymus using 60209-1-Ig (same clone as 60209-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse thymus tissue slide using 60209-1-Ig (CD7 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60209-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse thymus using 60209-1-Ig (same clone as 60209-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse thymus tissue slide using 60209-1-Ig (CD7 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60209-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human tonsillitis using 60209-1-Ig (same clone as 60209-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 60209-1-Ig (CD7 antibody) at dilution of 1:1600 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60209-1-PBS in a different storage buffer formulation.

• 

  IF Staining of human tonsillitis using 60209-1-Ig (same clone as 60209-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed human tonsillitis tissue using 60209-1-Ig (CD7 antibody), at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 60209-1-PBS in a different storage buffer formulation.

• 

  IF Staining of human tonsillitis using 60209-1-Ig (same clone as 60209-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed human tonsillitis tissue using 60209-1-Ig (CD7 antibody), at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 60209-1-PBS in a different storage buffer formulation.

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Recommended dilution

Application	Dilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

60209-1-PBS targets CD7 in IF, IHC, Indirect ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: CD7 fusion protein Ag1805
• Full Name: CD7 molecule
• Calculated Molecular Weight: 240 aa, 25 kDa
• GenBank Accession Number: BC009293
• Gene Symbol: CD7
• Gene ID (NCBI): 924
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

CD7 is a 40 kD type I transmembrane glycoprotein expressed on thymocytes and mature T cells. It is the most reliable clinical marker of T-cell acute lymphocytic leukemia.