CD69 Recombinant antibody

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  WB analysis using 84258-7-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 84258-7-RR (CD69 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  Affinity and Kinetic Characterization of 84258-7-RR

  Biolayer interferometry (BLl) kinetic assays of 84258-7-RR against Human CD69 were performed. The affinity constant is 0.776 nM.

CD69 Recombinant Antibody for WB, ELISA

Cat No. 84258-7-RR

Clone No.241552A2

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, ELISA

Synonyms

CD69 molecule, BL-AC/P26, BL AC/P26, AIM, Activation inducer molecule

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Tested Applications

• Positive WB detected in: BxPC-3 cells, Jurkat cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:4000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

84258-7-RR targets CD69 in WB, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: FusionProtein
• Full Name: CD69 molecule
• Calculated Molecular Weight: 23 kDa
• Observed Molecular Weight: 35 kDa
• GenBank Accession Number: NM_001781.2
• Gene Symbol: CD69
• Gene ID (NCBI): 969
• RRID: AB_3671805
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purfication
• UNIPROT ID: Q07108
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

CD69, also known as AIM, EA-1, Leu-23, and MLR3, is a type II transmembrane glycoprotein that belongs to the C-type lectin superfamily (PMID: 8340758; 7804122). CD69 is constitutively expressed by mature thymocytes, platelets, several subsets of tissue resident immune cells (including resident memory T cells and gamma delta T cells), and is inducibly expressed by activated T cells, B cells, natural killer (NK) cells, monocytes, neutrophils (PMID: 8100776; 28475283). CD69 has been identified as an early activation marker of lymphocytes and is commonly used as a marker of activated lymphocytes and NK cells (PMID: 28475283; 25759842). It is involved in the regulation of immune responses (PMID: 15745855).