CD69 Recombinant antibody, PBS Only (Detector)

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  Sandwich ELISA standard curve of MP01164-1

  Sandwich ELISA standard curve of MP01164-1, Human CD69 Recombinant Matched Antibody Pair - PBS only. 84258-5-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard Eg1791. 84258-1-PBS was HRP conjugated as the detection antibody. Range: 19.5-1250 pg/mL

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  Sample test of MP01164-1

  Daudi cells were cultured unstimulated or stimulated with 50 ng/mL PMA for 1 day. The mean CD69 concentration was determined to be 5.60 ng/mL in unstimulated Daudi cell extract based on a 2.7 mg/mL extract load and 83.4 ng/mL in stimulated Daudi cell extract based on a 3.1 mg/mL extract load.

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  Sample test of MP01164-1

  Serum of six individual healthy human donors was measured. The human CD69 concentration of detected samples was determined to be 1,504.2 pg/mL with a range of 592.9 - 2,756.6 pg/mL.

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  Cytometric bead array standard curve of MP01164-1

  Cytometric bead array standard curve of MP01164-1, CD69 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 84258-5-PBS. Detection antibody: 84258-1-PBS. Standard: Eg1791. Range: 0.156-20 ng/mL.

CD69 Uni-rAb^TM Recombinant Antibody for Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test

Cat No. 84258-1-PBS

Clone No.241552D1

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test

Synonyms

CD69 molecule, BL-AC/P26, BL AC/P26, AIM, Activation inducer molecule

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Product Information

84258-1-PBS targets CD69 as part of a matched antibody pair:

MP01164-1: 84258-5-PBS capture and 84258-1-PBS detection (validated in Cytometric bead array, Sandwich ELISA)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: Recombinant protein
• Full Name: CD69 molecule
• Calculated Molecular Weight: 23 kDa
• GenBank Accession Number: NM_001781.2
• Gene Symbol: CD69
• Gene ID (NCBI): 969
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: Q07108
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.