CD69 Monoclonal antibody

CD69 Monoclonal Antibody for WB, ELISA

Host / Isotype

Mouse / IgG3

Reactivity

Human

Applications

WB, ELISA

Conjugate

Unconjugated

CloneNo.

3D10A2

Cat no : 68827-1-Ig

Synonyms

Activation inducer molecule, AIM, BL AC/P26, CD69, CD69 molecule, CLEC2C, EA1, Early activation antigen CD69, GP32/28, MLR 3

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Validation Data Gallery

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  WB analysis using 68827-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 68827-1-Ig (CD69 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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Tested Applications

• Positive WB detected in: U-937 cells, THP-1 cells, HL-60 cells

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

68827-1-Ig targets CD69 in WB, ELISA applications and shows reactivity with Human samples.

• Tested Reactivity: Human
• Host / Isotype: Mouse / IgG3
• Class: Monoclonal
• Type: Antibody
• Immunogen: CD69 fusion protein Ag30963
• Full Name: CD69 molecule
• Calculated Molecular Weight: 23 kDa
• Observed Molecular Weight: 30 kDa
• GenBank Accession Number: BC007037
• Gene Symbol: CD69
• Gene ID (NCBI): 969
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

CD69, also known as AIM, EA-1, Leu-23, and MLR3, is a type II transmembrane glycoprotein that belongs to the C-type lectin superfamily (PMID: 8340758; 7804122). CD69 is constitutively expressed by mature thymocytes, platelets, and several subsets of tissue-resident immune cells (including resident memory T cells and gamma delta T cells), and is inducibly expressed by activated T cells, B cells, natural killer (NK) cells, monocytes, neutrophils (PMID: 8100776; 28475283). CD69 has been identified as an early activation marker of lymphocytes and is commonly used as a marker of activated lymphocytes and NK cells (PMID: 28475283; 25759842). It is involved in regulating immune responses (PMID: 15745855).