CD69 Polyclonal antibody

CD69 Polyclonal Antibody for WB, IHC, IF-P, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human and More (1)

Applications

WB, IHC, IF-P, ELISA

Conjugate

Unconjugated

Cat no : 10803-1-AP

Synonyms

CLEC2C, CD69 molecule, BL-AC/P26, BL AC/P26, AIM



Tested Applications

Positive WB detected inhuman peripheral blood platelets
Positive IHC detected inhuman tonsillitis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF-P detected inhuman tonsillitis tissue

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:3000
Immunohistochemistry (IHC)IHC : 1:2000-1:8000
Immunofluorescence (IF)-PIF-P : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

10803-1-AP targets CD69 in WB, IHC, IF-P, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Cited Reactivityhuman, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen CD69 fusion protein Ag1094
Full Name CD69 molecule
Calculated Molecular Weight 23 kDa
Observed Molecular Weight 28 kDa, 32 kDa
GenBank Accession NumberBC007037
Gene Symbol CD69
Gene ID (NCBI) 969
RRIDAB_2074965
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

CD69, also known as activation inducer molecule (AIM) or early activation antigen (EA1), is a cell surface glycoprotein expressed shortly after activation on T lymphocytes and other cells of haematopoietic origin, including natural killer cells. It is a disulfide-linked homodimer composed of two chains belonging to the type II integral protein with a C-type lectin domain. CD69 is involved in lymphocyte proliferation and functions as a signal-transmitting receptor in lymphocytes, natural killer cells, and platelets.

Protocols

Product Specific Protocols
WB protocol for CD69 antibody 10803-1-APDownload protocol
IHC protocol for CD69 antibody 10803-1-APDownload protocol
IF protocol for CD69 antibody 10803-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
IHC

Cancer Commun (Lond)

Targeting autophagy overcomes cancer-intrinsic resistance to CAR-T immunotherapy in B-cell malignancies

Authors - Lu Tang
humanIF

iScience

Highly multiplexed spatial analysis identifies tissue-resident memory T cells as drivers of ulcerative and immune checkpoint inhibitor colitis

Authors - Mick J M van Eijs
humanIF

Expert Rev Clin Immunol

Juxtaposition of IL-1β and IFN-γ expression and apoptosis of keratinocytes in adult-onset Still's disease.

Authors - Shijia Rao
IF

Cell Rep

Longitudinal analyses reveal distinct immune response landscapes in lung and intestinal tissues from SARS-CoV-2-infected rhesus macaques.

Authors - Huiwen Zheng
humanIHC

Oncoimmunology

Development and validation of an interferon signature predicting prognosis and treatment response for glioblastoma.

Authors - Chen Zhu
mouseFC

J Nanobiotechnology

Optogenetic engineered umbilical cord MSC-derived exosomes for remodeling of the immune microenvironment in diabetic wounds and the promotion of tissue repair

Authors - Xin Zhao