Anti-Mouse CD68 Rabbit Recombinant Antibody

CD68 Recombinant Antibody for FC (Intra)

Host / Isotype

Rabbit / IgG

Reactivity

Mouse

Applications

FC (Intra)

Conjugate

Unconjugated

CloneNo.

230504G5

Cat no : 98029-1-RR

Synonyms

CD68, CD68 antigen, gp110, Scard1, 230504G5

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Validation Data Gallery

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  FC experiment of mouse peritoneal macrophages using 98029-1-RR

  1x10^6 mouse peritoneal macrophages were intracellularly stained with 0.25 ug Anti-Mouse CD68 Rabbit Recombinant Antibody (98029-1-RR, Clone: 230504G5) or Isotype Control and PE-conjugated Goat Anti-Rabbit IgG. Cell were then stained with APC Anti-Mouse CD11b. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

• 

  FC experiment of mouse peritoneal macrophages using 98029-1-RR

  1x10^6 mouse peritoneal macrophages were intracellularly stained with 0.25 ug Anti-Mouse CD68 Rabbit Recombinant Antibody (98029-1-RR, Clone: 230504G5) (red) or Isotype Control (blue), and PE-conjugated Goat Anti-Rabbit IgG. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Tested Applications

• Positive FC detected in: mouse peritoneal macrophages

Recommended dilution

Application	Dilution
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 98029-1-RR is CD68 Fusion Protein expressed in E. coli.

• Tested Reactivity: Mouse
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: Fusion Protein
• Full Name: CD68 antigen
• Calculated Molecular Weight: 35 kDa
• GenBank Accession Number: NM_001291058.1
• Gene Symbol: Cd68
• Gene ID (NCBI): 12514
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purfication
• Storage Buffer: PBS with 0.09% sodium azide.
• Storage Conditions: Store at 2-8°C. Stable for one year after shipment.

Background Information

Mouse CD68 (also known as macrosialin) is a type I transmembrane glycoprotein that is highly and specifically expressed by mouse tissue macrophages, and to a lesser extent by dendritic cells. It belongs to the lysosomal/endosomal-associated membrane glycoprotein (LAMP) family and primarily localizes to lysosomes and endosomes with a smaller fraction circulating to the cell surface. CD68 is also a member of the scavenger receptor family. It may play a role in phagocytic activities of tissue macrophages.